Conditioned cell culture medium compositions and methods of use
First Claim
1. A pharmaceutical composition comprising conditioned cell culture medium, said medium comprising medium which has previously supported the growth of eukaryotic cells cultured in three-dimensions, and a pharmaceutical carrier.
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Accused Products
Abstract
Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. The medium may be conditioned by stromal cells, parenchymal cells, mesenchymal stem cells, liver reserve cells, neural stem cells, pancreatic stem cells and/or embryonic stem cells. Additionally, the cells may be genetically modified. A three-dimensional tissue construct is preferred. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, formulated with a salve or ointment for topical applications, or, for example, made into or added to surgical glue to accelerate healing of sutures following invasive procedures. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.
99 Citations
60 Claims
- 1. A pharmaceutical composition comprising conditioned cell culture medium, said medium comprising medium which has previously supported the growth of eukaryotic cells cultured in three-dimensions, and a pharmaceutical carrier.
- 15. A food substance with enhanced nutritional content comprising conditioned cell culture medium, said medium comprising medium which has previously supported the growth of eukaryotic cells together with a separate food item for consumption by a mammal.
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17. A nutritional supplement comprising conditioned cell culture medium, said medium comprising medium which has previously supported the growth of eukaryotic cells, together with a carrier suitable for human consumption and where said carrier is in the form of a liquid, tablet or capsule.
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18. A method of making a pharmaceutical composition comprising:
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(a) culturing eukaroytic cells in three-dimensions in a cell culture medium sufficient to meet the nutritional needs required to grow the cells in vitro;
(b) culturing the cells in vitro until the cell culture medium contains a desired level of extracellular products so that a conditioned medium is formed;
(c) removing the conditioned medium from the cells used to condition the medium; and
(d) combining the conditioned medium with a pharmaceutical carrier. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35)
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- 36. A method for improved wound or burn healing comprising administering to a patient in need of wound or burn healing treatment a composition comprising cell culture medium, said medium comprising medium which has previously supported the growth of eukaryotic cells cultured in three-dimensions, together with a separate therapeutic component so that the patient exhibits a reduction in the amount of traumatized tissue or scar tissue and further exhibits improved regeneration of healthy tissue at the wound site.
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55. A method for the correction of a cosmetic defect comprising administering to a person a composition comprising conditioned cell culture medium, said medium comprising medium which has previously supported the growth of eukaryotic cells cultured in three-dimensions, together with a component useful for cosmetic purposes, so that the person exhibits a cosmetic improvement.
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56-57. -57. (canceled)
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58. A method for the stimulation of hair growth comprising topically administering to a person a composition comprising conditioned cell culture medium, said medium comprising medium which has previously the growth of eukaryotic cells cultured in three dimensions, together with a topical carrier, so that the person exhibits an improved stimulation of hair growth.
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59. (canceled)
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60. A method for isolating collagen, comprising:
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(a) precipitating procollagen from medium conditioned by a three-dimensional culture at high salt conditions under neutral pH conditions;
(b) removing the propeptides under acidic conditions so that the triple helix of the collagen remains intact; and
(c) precipitating the collagen at high salt concentrations.
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Specification