Detection device and methods associated therewith

• US 20070116600A1
• Filed: 06/23/2006
• Published: 05/24/2007
• Est. Priority Date: 06/23/2005
• Status: Abandoned Application

• Current Assignee: BioVeris Corporation (Roche Holding AG)
• Original Assignee: BioVeris Corporation (Roche Holding AG)
• Inventors: Davis, Charles, Kochar, Manish, Cook, Richard, Manteuffel, Richard, Leland, Jonathan
• Application Number:
  US11/474,099
• Publication Number:
  US 20070116600A1
• Time in Patent Office: Days
• US Class Current: 422/65
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2300/0858 Side walls
• CPC Class Codes: B01L 3/5082 Test tubes per se
• CPC Class Codes: G01N 2035/0436 with pre-packaged reagents, i.e. test-packs
• CPC Class Codes: G01N 2035/0493 Locating samples; identifying different tube sizes
• CPC Class Codes: G01N 2035/1025 Fluid level sensing
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 35/00 Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor

Abstract

Detection devices and methods associated therewith are provided. In some embodiments, the detection system can be adapted to measure one or more analytes of interest possibly present in a sample through the use of binding reactions.

Claims

1. A biological detection system used to measure one or more analytes of interest possibly present in a sample through the use of binding reactions comprising:

a detector configured to detect a label used in the binding reactions;

a holder configured to hold at least one multi-well reagent container;

at least one multi-well reagent container comprising the label and binding reagents for a plurality of binding reactions;

a probe configured to distribute a portion of a sample into at least one of the multi-well reagent containers;

one pump fluidically connected to the probe; and

a liquid-level detector for determining the presence and/or amount of liquid in at least one of a sample container and the multi-well reagent container.

2. The system of claim 1, further comprising an electrical energy source and an electrode, both suitable for initiating electrochemiluminescence.

3. The system of claim 1, further comprising a flow cell, wherein the flow cell comprises the detector and the flow cell fluidically connected to the probe and pump.

6. The system of claim 1, further comprising a filter configured to filter the sample to form a filtrate, wherein the filtrate is used in the binding reactions.

33. The system of claim 1, wherein the holder for the at least one multi-well reagent container has a capacity to hold at least 2 of the containers.

34. The system of claim 33, wherein the holder for the at least one multi-well reagent container has a capacity to hold at least 6 of the containers.

38. The system of claim 1, wherein the multi-well reagent container has identical reagents in at least 2 wells.

53. The system of claim 1, wherein the multi-well reagent container comprises a well that holds binding reagents specific for at least 2 of the analytes of interest.

56. The system of claim 53, wherein the multi-well reagent container comprises a well that holds binding reagents specific for at least 4 of the analytes of interest.

63. The system of claim 1, wherein the binding reagents comprise magnetizable beads;

and wherein the system further comprises at least 2 magnetic capture zones.

64. The system of claim 63, wherein at least one of the magnetic capture zones is located fluidically between the tip of the probe and the flow cell.

85. The system of claim 2, further comprising:

a temperature control system used to regulate the temperature of the at least one multi-well reagent container and/or a location of the label when detected by the detector;

an ECL coreactant selected from piperazine-1,4-bis(2-ethanesulfonic acid);

tri-n-propylamine;

N,N,N′

,N′

-Tetrapropyl-1,3-diaminopropane;

or salts thereof; and

an agitator configured to agitate the at least one multi-well reagent container;

wherein the label comprises a ruthenium chelate, an osmium chelate, or a mixture of both, wherein the system comprises one probe, the probe being part of the liquid level detector;

wherein the binding reagents are dry and comprise magnetizable beads having a diameter ranging from about 0.4 microns to about 3 microns, and wherein the detector is a light detector.

106. The system of claim 3, wherein the flow cell is configured to measure radioactivity, optical absorbance, magnetic materials, magnetizable materials, light scattering, optical interference, surface plasmon resonance, luminescence, or a combination of any of the foregoing.

4-5. -5. (canceled)

7-32. -32. (canceled)

35-37. -37. (canceled)

39-52. -52. (canceled)

54-55. -55. (canceled)

57-62. -62. (canceled)

65-84. -84. (canceled)

86-91. -91. (canceled)

92. A method to measure an analyte of interest possibly present in liquid in a sample container comprising:

(a) forming a composition in a well comprising a sample of optionally processed liquid from the sample container and binding reagents comprising a plurality of magnetizable beads, a plurality of labels, and a plurality of reagents specific for the one or more analytes of interest;

(b) incubating the composition to form complexes among the label, analyte of interest, and the plurality of magnetizable beads;

(c) separating any non-complexed label composition and sample matrix from the complexed label using a method comprising;

(i) aspirating the incubated composition from the well;

(ii) capturing the magnetizable beads with a magnet; and

(iii) dispensing the non-captured label composition into a waste location;

(d) releasing the captured magnetizable beads;

(e) transporting the magnetizable beads to a measurement zone; and

(f) detecting the complexed label to measure the concentration of the analyte of interest.

98. The method of claim 92, wherein the binding reagents are dry.

99. The method of claim 98, wherein the dry binding reagents are rehydrated solely by the sample.

100. The method of claim 92, further comprising

(iv) dispensing additional liquid into the waste location after step (iii) and before step (d).

101. The method of claim 92, wherein the waste location is the well.

93-97. -97. (canceled)

102-105. -105. (canceled)

107-119. -119. (canceled)

120. A biological detection system used to measure one or more analytes of interest possibly present in a sample through the use of binding reactions comprising:

a detector configured to detect a label used in the binding reactions;

a holder configured to hold at least one multi-well reagent container;

a probe, the probe configured to at least distribute a known amount of sample into at least one of the at least one multi-well reagent containers;

a pump, fluidically connected to the probe; and

2 or more magnetic capture zones fluidically connected and configured to collect and release magnetizable beads.

121. The system of claim 120, wherein at least one of the 2 or more magnetic capture zones is located fluidically between the tip of the probe and a location wherein the label is detected by the detector.

122. The system of claim 120, wherein the magnetic capture zones are configured to collect and release magnetizable beads that have a diameter ranging from about 0.4 microns to about 3 microns.

123. The system of claim 120, further comprising an electrical energy source and an electrode, both suitable for initiating electrochemiluminescence.

126. The system of claim 120, further comprising a filter configured to filter the sample to form a filtrate, wherein the filtrate is used in the binding reactions.

149. The system of claim 123, further comprising:

a temperature control system used to regulate the temperature near the at least one multi-well reagent container and/or a location of the label when detected by the detector;

an ECL coreactant selected from piperazine-1,4-bis(2-ethanesulfonic acid);

tri-n-propylamine;

N,N,N′

,N′

-Tetrapropyl-1,3-diaminopropane;

or salts thereof; and

an agitator configured to agitate the at least one multi-well reagent container;

wherein the label comprises a ruthenium chelate, an osmium chelate, or a mixture of both, wherein the system comprises one probe, the probe being part of the liquid level detector;

the magnetic capture zones are configured to collect and release magnetizable beads that have a diameter ranging from about 0.4 microns to about 3 microns, and wherein the detector is a light detector.

124-125. -125. (canceled)

127-148. -148. (canceled)

150-152. -152. (canceled)

153. A multi-well reagent container comprising:

one or more vessels comprising binding reagents specific for an analyte of interest, a vessel opening through which the binding reagents enter and leave the vessel, and a vessel bottom which is the most distant part of the vessel from the vessel opening; and

a receptacle adapted to receive each of said one or more vessels comprising zero or more reagent cavities;

wherein one or more vessels are physically separate parts that are installed into the receptacle and are held in the receptacle via an attachment retention member;

wherein the number of wells is considered to be the sum of the number of reagent cavities and number of vessels; and

wherein the number of wells is 2 or more.

156. The multi-well reagent container of claim 153, wherein the attachment retention member is a snap fit.

158. The multi-well reagent container of claim 153, wherein the attachment retention member is configured so that the vessel bottom can move a greater distance than the vessel opening.

159. The multi-well reagent container of claim 153, wherein the vessel bottom forms part of the exterior of the multi-well reagent container.

160. The multi-well reagent container of claim 153, wherein the vessel opening is covered by a seal that is pierceable by a probe.

163. The multi-well reagent container of claim 153, wherein the number of vessels ranges from 2 to 36.

154-155. -155. (canceled)

157. (canceled)

161-162. -162. (canceled)

164-172. -172. (canceled)