Biomolecule detection device, mobile phone for biomolecule detection, and biomolecule detection method

• US 20070131552A1
• Filed: 12/06/2006
• Published: 06/14/2007
• Est. Priority Date: 12/08/2005
• Status: Active Grant

• Current Assignee: Electronics and Telecommunications Research Institute
• Original Assignee: Electronics and Telecommunications Research Institute
• Inventors: Pyo, Hyeon, Jung, Moon, Park, Se, Shin, Dong
• Granted Patent: US 7,828,949 B2
• Time in Patent Office: Days
• US Class Current: 204/456
• CPC Class Codes: A61B 5/0002 Remote monitoring of patients using telemetry, e.g. transmission of vital signals via a communication network A61B5/07 takes precedence; transmission systems for measured values G08C; transmission H04B; transmission of digital information H04L; wireless communication networks H04W
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/544 the carrier being organic
• CPC Class Codes: G01N 33/548 Carbohydrates, e.g. dextran

Abstract

Provided are a biomolecule detection device, a mobile phone for biomolecule detection, and a biomolecule detection method. The biomolecule detection device includes an electrophoresis unit comprising an electrophoretic gel filtering erythrocytes and leukocytes in blood and transferring proteins and DNAs in the blood, and at least one type of a probe biomolecule, immobilized in the electrophoretic gel, reacting with a target biomolecule; a conversion unit converting a result of a reaction between the target biomolecule and the probe biomolecule to an electrical signal; and a lead-out unit receiving, converting, and transmitting the electrical signal.

Claims

1. A biomolecule detection device comprising:

an electrophoresis unit comprising an electrophoretic gel, and at least one type of a probe biomolecule, immobilized in the electrophoretic gel, reacting with a target biomolecule;

a conversion unit converting a result of a reaction between the target biomolecule and the probe biomolecule to an electrical signal; and

a lead-out unit receiving, converting, and transmitting the electrical signal.

2. The biomolecule detection device of claim 1, wherein the electrophoresis unit further comprises an electrode generating an electric field necessary for electrophoresis.

3. The biomolecule detection device of claim 1, wherein the electrophoresis unit further comprises a glass fiber or a polymer material which is disposed in the electrophoretic gel and immobilizes the probe biomolecule.

4. The biomolecule detection device of claim 1, wherein the electrophoresis unit further comprises a microchannel comprising the electrophoretic gel and the probe biomolecule.

5. The biomolecule detection device of claim 1, wherein the electrophoretic gel is an agarose gel or a polyacrylamide gel.

6. The biomolecule detection device of claim 1, wherein the target biomolecule and the probe biomolecule are each a nucleic acid or a protein.

7. The biomolecule detection device of claim 6, wherein the nucleic acid is selected from the group consisting of DNAs, RNAs, PNAs, LNAs, and hybrids thereof.

8. The biomolecule detection device of claim 6, wherein the protein is selected from the group consisting of enzymes, substrates, antigens, antibodies, ligands, aptamers, and receptors.

9. The biomolecule detection device of claim 1, wherein the target biomolecule and the probe biomolecule are respectively an antigen and an antibody or a target DNA and a probe DNA.

10. The biomolecule detection device of claim 1, wherein the electrophoresis unit further comprises two substrates facing each other, and the electrophoretic gel and the probe biomolecule are interposed between the two substrates.

11. The biomolecule detection device of claim 10, wherein the two substrates are made of a transparent material.

12. The biomolecule detection device of claim 1, wherein the conversion unit is a photodiode array.

13. The biomolecule detection device of claim 12, wherein the photodiode array is a photodiode array capable of detecting visible light or UV light.

14. A mobile phone for biomolecule detection, comprising:

an electrophoresis unit comprising an electrophoretic gel, and at least one type of a probe biomolecule, immobilized in the electrophoretic gel, reacting with a target biomolecule;

a conversion unit converting a result of a reaction between the target biomolecule and the probe biomolecule to an electrical signal;

a lead-out unit receiving, converting, and transmitting the electrical signal;

a power supply unit supplying power to the electrophoresis unit, the conversion unit, and the lead-out unit;

an optical source irradiating light to the electrophoresis unit; and

a transmitter transmitting information received from the lead-out unit wirelessly.

15. The mobile phone of claim 14, wherein the optical source is an LCD screen of the mobile phone.

16. The mobile phone of claim 15, wherein the optical source further comprises a UV light source.

17. A method of detecting a target biomolecule using a mobile phone for biomolecule detection, the mobile phone comprising an electrophoresis unit comprising an electrophoretic gel, and at least one type of a probe biomolecule, immobilized in the electrophoretic gel, reacting with a target biomolecule;

a conversion unit converting a result of a reaction between the target biomolecule and the probe biomolecule to an electrical signal;

a lead-out unit receiving, converting, and transmitting the electrical signal;

a power supply unit supplying power to the electrophoresis unit, the conversion unit, and the lead-out unit;

an optical source irradiating light to the electrophoresis unit; and

a transmitter transmitting information received from the lead-out unit wirelessly,

the method comprising;

loading a sample on the electrophoresis unit to perform electrophoresis;

irradiating light to the electrophoresis unit using the optical source; and

transmitting the detection result wirelessly.

18. The method of claim 17, further comprising mixing the sample with a solution comprising a molecule capable of binding with the target biomolecule and absorbing light emitted from the optical source or a molecule capable of binding with the target biomolecule, absorbing light emitted from the optical source, and emitting light with a predetermined wavelength, prior to loading the sample on the electrophoresis unit.

19. The method of claim 17, wherein the sample is selected from the group consisting of saliva, urine, blood, serum, cell culture, and drinking water.