Human antibodies that bind human TNFa
First Claim
1. An antigen binding portion of an antibody that binds human TNFα
- comprising a CDR3 domain comprising an amino acid sequence of SEQ ID NO;
3, or modified from SEQ ID NO;
3 by a single alanine substitution at position 1, 4, 5, 7 or 8 or by one to five conservative amino acid substitutions at positions 1, 3, 4, 6, 7, 8.
6 Assignments
0 Petitions
Accused Products
Abstract
Human antibodies, preferably recombinant human antibodies, that specifically bind to human tumor necrosis factor α (hTNFα) are disclosed. These antibodies have high affinity for hTNFα (e.g., Kd=10−8 M or less), a slow off rate for hTNFα dissociation (e.g., Koff=10−3 sec−1 or less) and neutralize hTNFα activity in vitro and in vivo. An antibody of the invention can be a full-length antibody or an antigen-binding portion thereof. The antibodies, or antibody portions, of the invention are useful for detecting hTNFα and for inhibiting hTNFα activity, e.g., in a human subject suffering from a disorder in which hTNFα activity is detrimental. Nucleic acids, vectors and host cells for expressing the recombinant human antibodies of the invention, and methods of synthesizing the recombinant human antibodies, are also encompassed by the invention.
97 Citations
9 Claims
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1. An antigen binding portion of an antibody that binds human TNFα
- comprising a CDR3 domain comprising an amino acid sequence of SEQ ID NO;
3, or modified from SEQ ID NO;
3 by a single alanine substitution at position 1, 4, 5, 7 or 8 or by one to five conservative amino acid substitutions at positions 1, 3, 4, 6, 7, 8. - View Dependent Claims (2, 3, 5)
- comprising a CDR3 domain comprising an amino acid sequence of SEQ ID NO;
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4. An antigen binding portion of an antibody that binds human TNFα
- comprising a CDR3 domain comprising an amino acid sequence of SEQ ID NO;
4, or modified from SEQ ID NO;
4 by a single alanine substitution at position 2, 3, 4, 5, 6, 8, 9, 10 or 11 or by one to five conservative amino acid substitutions at positions 2, 3, 4, 5, 6,8,9,10, 11 and/or 12. - View Dependent Claims (6)
- comprising a CDR3 domain comprising an amino acid sequence of SEQ ID NO;
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7. A dAb that binds human TNFα
- comprising a CDR3 domain comprising an amino acid sequence of SEQ ID NO;
4, or modified from SEQ ID NO;
4 by a single alanine substitution at position 2, 3, 4, 5, 6, 8, 9, 10 or 11 or by one to five conservative amino acid substitutions at positions 2, 3, 4, 5, 6, 8, 9, 10, 11 and/or 12.
- comprising a CDR3 domain comprising an amino acid sequence of SEQ ID NO;
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8. A dAb that binds human TNFα
- comprising a CDR2 domain comprising an amino acid sequence of SEQ ID NO;
6.
- comprising a CDR2 domain comprising an amino acid sequence of SEQ ID NO;
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9. A dAb that binds human TNFα
- comprising a CDR1 domain comprising an amino acid sequence of SEQ ID NO;
8.
- comprising a CDR1 domain comprising an amino acid sequence of SEQ ID NO;
Specification