DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS

  • US 20070254359A1
  • Filed: 04/18/2007
  • Published: 11/01/2007
  • Est. Priority Date: 04/28/2006
  • Status: Active Grant
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First Claim
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1. A method for differentiating pluripotent stem cells into cells expressing markers characteristic of the pancreatic endocrine lineage, comprising the steps of:

  • a. Culturing the pluripotent stem cells, b. Differentiating the pluripotent stem cells into cells expressing markers characteristics of the definitive endoderm lineage, by treating the pluripotent cells with activin A, c. Differentiating the cells expressing markers characteristics of the definitive endoderm lineage into cells expressing markers characteristics of the pancreatic endoderm lineage, by treating the cells expressing markers characteristics of the definitive endoderm lineage with at least one fibroblast growth factor, or with retinoic acid and at least one fibroblast growth factor, and d. Differentiating the cells expressing markers characteristics of the pancreatic endoderm lineage into cells expressing markers characteristics of the pancreatic endocrine lineage, by treating the cells expressing markers characteristics of the pancreatic endoderm lineage with a gamma secretase inhibitor.

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