Rapid analyte measurement assay

• US 20080000780A1
• Filed: 06/30/2006
• Published: 01/03/2008
• Est. Priority Date: 06/30/2006
• Status: Active Grant

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Inventors: Tonks, Simon
• Granted Patent: US 7,699,973 B2
• Time in Patent Office: Days
• US Class Current: 205/792
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771

Abstract

The present the invention provides methods, devices and systems for rapidly measuring analytes within a biological sample.

Claims

1. A method of measuring the concentration of a target analyte within a biological sample, the method comprising:

applying the biological sample to an electrochemical cell having a redox reagent system comprising a target analyte-dependent redox couple;

applying a square wave excitation voltage across the electrochemical cell; and

measuring a response current of the electrochemical cell, wherein the response current is representative of the concentration of the target analyte within the biological sample.

2. The method of claim 1, wherein measuring the current response is performed within less than about 1 second.

3. The method of claim 1, wherein the square wave excitation voltage is applied at a frequency not less than about 100 Hz and scanned across range of about 1 volt.

4. The method of claim 1, wherein the target analyte is glucose and the biological sample is blood.

5. The method of claim 1, wherein the square wave is superimposed over a potential step function to provide a potential waveform.

6. A method of measuring the concentration of a target analyte within a biological sample, the method comprising:

applying the biological sample to an electrochemical cell having a redox reagent system comprising a first redox couple and at least one secondary redox couple, wherein the first redox couple is selected to mediate the target analyte and the at least one secondary redox couple is selected so as not to interfere with the target analyte mediation;

applying a square wave excitation voltage across the electrochemical cell;

measuring a response current of the electrochemical cell, wherein the response current has a primary peak associated with mediation of the target analyte and one or more secondary peaks associated with mediation of at least one non-interfering secondary redox couple; and

determining the concentration of the target analyte within the biological sample based on the primary and secondary current peaks.

7. The method of claim 6, wherein determining the concentration of the target analyte comprises applying a correction factor to the value of the measurement from the primary peak based on the value of the measurement from the secondary peak.

8. The method of claim 7, wherein determining the concentration of the target analyte comprises compensating for the effect that hematocrit in a blood sample has on the value of the primary peak measurement.

9. The method of claim 6, wherein the first redox couple comprises a mediator for the glucose reaction with a glucose specific enzyme, and at least one secondary redox couple comprises a redox couple that does not interfere with the primary glucose reaction or it'"'"'s mediation.

10. The method of claim 7, wherein determining the concentration of the target analyte comprises compensating for the effect that the temperature of the biological sample has on the value of the primary peak measurement.

11. The method of claim 6, wherein the square wave is superimposed over a potential step function to provide a potential waveform.

12. A device comprising:

a test strip comprising an electrochemical cell having a redox reagent system comprising a target analyte-dependent redox couple; and

a meter configured for applying a square wave excitation voltage across the electrochemical cell, wherein the test strip is configured for electrical engagement with the meter.

13. The device of claim 12, wherein the target analyte is glucose.

14. The device of claim 12, wherein the electrochemical cell further comprises at least one secondary redox couple, wherein the first redox couple is selected to mediate the target analyte and the at least one secondary redox couple is selected so as not to interfere with the target analyte reaction.

15. The device of claim 12, wherein the target analyte is glucose and the secondary redox couple signal is used to compensate for hematocrit.

16. The device of claim 12, wherein the first redox couple comprises a glucose mediator and the at least one secondary redox couple comprises a redox couple that does not interfere with the primary glucose reaction or it'"'"'s mediation.

17. A system comprising:

an electrochemical cell having a redox reagent system comprising a target analyte-dependent redox couple; and

a meter configured for applying a square wave excitation voltage across the electrochemical cell, wherein the meter is in electrical communication with the electrochemical cell.

18. The system of claim 17, wherein the target analyte is glucose.

19. The system of claim 17, wherein the electrochemical cell further comprises at least one secondary redox couple, wherein the first redox couple is selected to mediate the target analyte and the at least one secondary redox couple is selected so as not to interfere with the reaction of the target analyte.

20. The system of claim 17, wherein the target analyte is glucose and the secondary redox couple signal is used to compensate for sample temperature.

21. The system of claim 17, wherein the first redox couple comprises a glucose mediator and at least one secondary redox couple comprises a redox couple that does not interfere with the primary glucose reaction or it'"'"'s mediation.