Biosensor Utilizing Pigment-Synthesizing Gene Of A Purple Non-Sulfur Bacterium And A Method For Preparing Such A Biosensor
First Claim
1. A biosensor for the detection of a specific chemical substance, characterized in that it comprises a recombinant purple non-sulfur bacterium wherein sensor vector is introduced into a pigment variant of a purple non-sulfur bacterium being deficient in gene coding for a spheroidene monooxygenase enzyme, and that the sensor vector is a sensor vector where inductive promoter showing a specific response to a specific chemical substance is connected in an operable manner to the upstream region of gene coding for the spheroidene monooxygenase enzyme.
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Abstract
A biosensor where measurement is able to be conducted by simpler operations, cost is less expensive and specific chemical substance is able to be visually detected is provided. In accordance with the present invention, there is provided a biosensor for the detection of a specific chemical substance, characterized in that it comprises a recombinant purple non-sulfur bacterium wherein sensor vector is introduced into a pigment variant of a purple non-sulfur bacterium being deficient in gene coding for a spheroidene monooxygenase enzyme, and that the sensor vector is a sensor vector where inductive promoter showing a specific response to a specific chemical substance is connected in an operable manner to the upstream region of gene coding for the spheroidene monooxygenase enzyme.
5 Citations
12 Claims
- 1. A biosensor for the detection of a specific chemical substance, characterized in that it comprises a recombinant purple non-sulfur bacterium wherein sensor vector is introduced into a pigment variant of a purple non-sulfur bacterium being deficient in gene coding for a spheroidene monooxygenase enzyme, and that the sensor vector is a sensor vector where inductive promoter showing a specific response to a specific chemical substance is connected in an operable manner to the upstream region of gene coding for the spheroidene monooxygenase enzyme.
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7. A method for the preparation of a biosensor for detection of a specific chemical substance, characterized in that it comprises the steps of:
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preparing a pigment variant of a purple non-sulfur bacterium being deficient in gene coding for spheroidene monooxygenase enzyme;
cloning an open reading frame of gene coding for spheroidene monooxygenase enzyme being deficient in promoter on vector;
connecting an inductive promoter showing a specific response to a specific chemical substance in an operable manner to the upstream region of gene coding for spheroidene monooxygenase enzyme in the vector to construct a sensor vector; and
introducing the sensor vector into the pigment variant of a purple non-sulfur bacterium to prepare a recombinant purple non-sulfur bacterium. - View Dependent Claims (8, 9, 10, 11, 12)
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Specification