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Device and method for multiple analyte detection

  • US 20080102461A1
  • Filed: 06/11/2007
  • Published: 05/01/2008
  • Est. Priority Date: 04/03/1996
  • Status: Active Grant
First Claim
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1. A method comprising:

  • pre-loading a first unlabeled oligonucleotide into a chamber;

    pre-loading a second unlabeled oligonucleotide into the chamber;

    introducing a target nucleic acid sequence into the chamber, after the pre-loading of the first unlabeled oligonucleotide and of the second unlabeled oligonucleotide;

    reacting the target nucleic acid sequence with both the first unlabeled oligonucleotide and the second unlabeled oligonucleotide in the chamber, to form a reaction product in the chamber; and

    reacting the reaction product, in the chamber, with a non-immobilized optically detectable detection reagent that is specific for the target nucleic acid sequence.

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