Device and method for multiple analyte detection
First Claim
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1. A method comprising:
- pre-loading a first unlabeled oligonucleotide into a chamber;
pre-loading a second unlabeled oligonucleotide into the chamber;
introducing a target nucleic acid sequence into the chamber, after the pre-loading of the first unlabeled oligonucleotide and of the second unlabeled oligonucleotide;
reacting the target nucleic acid sequence with both the first unlabeled oligonucleotide and the second unlabeled oligonucleotide in the chamber, to form a reaction product in the chamber; and
reacting the reaction product, in the chamber, with a non-immobilized optically detectable detection reagent that is specific for the target nucleic acid sequence.
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Abstract
The invention is directed to a method and device for simultaneously testing a sample for the presence, presence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.
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11 Claims
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1. A method comprising:
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pre-loading a first unlabeled oligonucleotide into a chamber; pre-loading a second unlabeled oligonucleotide into the chamber; introducing a target nucleic acid sequence into the chamber, after the pre-loading of the first unlabeled oligonucleotide and of the second unlabeled oligonucleotide; reacting the target nucleic acid sequence with both the first unlabeled oligonucleotide and the second unlabeled oligonucleotide in the chamber, to form a reaction product in the chamber; and reacting the reaction product, in the chamber, with a non-immobilized optically detectable detection reagent that is specific for the target nucleic acid sequence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification