Emulsion compositions

• US 20080187968A1
• Filed: 03/28/2008
• Published: 08/07/2008
• Est. Priority Date: 11/16/2001
• Status: Active Grant

• Current Assignee: UK Research and Innovation
• Original Assignee: Medical Research Council (Government of United Kingdom)
• Inventors: Holliger, Phillip, Ghadessy, Farid
• Granted Patent: US 7,655,435 B2
• Time in Patent Office: Days
• US Class Current: 435/91.1
• CPC Class Codes: A61K 47/24 containing atoms other than carbon, hydrogen, oxygen, halogen, nitrogen or sulfur, e.g. cyclomethicone or phospholipids
• CPC Class Codes: A61K 47/26 Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
• CPC Class Codes: A61K 47/44 Oils, fats or waxes according to two or more groups of A61K47/02-A61K47/42; Natural or modified natural oils, fats or waxes, e.g. castor oil, polyethoxylated castor oil, montan wax, lignite, shellac, rosin, beeswax or lanolin synthetic glycerides, e.g. medium-chain triglycerides, A61K47/14
• CPC Class Codes: A61K 9/107 Emulsions ; Emulsion preconcentrates; Micelles composition of emulsions A61K47/00
• CPC Class Codes: A61K 9/1676 having a drug-free core with discrete complete coating layer containing drug adsorbates of liquid drug formulations on inert powders without simultaneous granulation step A61K9/141; with further drug-free outer coating A61K9/5078; drug conjugated to non-active particles A61K47/6921
• CPC Class Codes: C12N 15/1075 by coupling phenotype to genotype, not provided for in other groups of this subclass
• CPC Class Codes: C12Q 1/6844 Nucleic acid amplification reactions
• CPC Class Codes: C12Q 2527/125 Specific component of sample, medium or buffer

Abstract

An emulsion is useful in allowing a wide variety of gene products to be expressed via eukaryotic in vitro expression. The emulsion comprises a silicone based surfactant, a hydrophobic phase and a hydrophilic phase; wherein the hydrophilic phase comprises a plurality of compartments containing a functional in vitro eukaryotic expression system.

Claims

1-22. -22. (canceled)

23. A method of obtaining a gene product comprising:

forming an emulsion comprising a surfactant, a hydrophobic phase and a hydrophilic phase comprising a plurality of microcapsules containing a functional in vitro eukaryotic expression system, wherein the surfactant is a chemically inert silicone-based surfactant, allowing the gene product to be expressed by the expression system and, optionally, purifying the gene product.

24. The method of claim 23, wherein the silicone-based surfactant is a silicone copolymer.

25. The method of claim 24, wherein, the silicone-based surfactant is polysiloxane-polycetyl-polyethylene glycol copolymer.

26. The method of claim 23, wherein the surfactant is provided at a v/v concentration in the oil phase of 4%.

27. The method of claim 23, wherein the emulsion comprises an in vitro eukaryotic expression system.

28. The method of claim 27, wherein the eukaryotic expression system comprises all or part of a mammalian cell lysate.

29. The method of claim 28, wherein the lysate is a reticulocyte lysate.

30. The method of claim 28, wherein the lysate is a rabbit reticulocyte lysate.

31. The method of claim 23, wherein the expression and/or the activity of the gene product within the emulsion is at a level of at least 1% of that of the gene product achievable by the expression system prior to formation of the emulsion.

32. The method of claim 31 wherein the expression and/or the activity of the gene product within the emulsion is at a level of at least 10%, at least 20%, or at least 30% of that of the gene product achievable with the expression system prior to formation of the emulsion.

33. The method of claim 23 wherein the emulsion is stable during incubation at 30°

C. for at least one hour.

34. The method of claim 23, wherein the hydrophobic phase comprises a mineral oil.