Stabilization of Enzymes
First Claim
1. A process for producing enzyme particles, which process comprising includesproviding an emulsion of droplets of a first liquid phase dispersed in a second liquid phase, with the one liquid phase being a hydrophilic phase and the other liquid phase being a hydrophobic phase which is immiscible with the hydrophilic phase, and with enzyme molecules being located at or within interfacial boundaries of the droplets and the second liquid phase;
- cross-linking the enzyme molecules of the respective droplets so that individual enzyme particles, which are stable and in which the enzymes are immobilized with a majority of active sites of the enzymes being orientated either internally or externally, are formed from individual droplets; and
recovering the individual enzyme particles from the second liquid phase.
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Abstract
A process for producing enzymes structures includes providing an emulsion of droplets of a first liquid phase dispersed in a second liquid phase. The one liquid phase is a hydrophilic phase, while the other liquid phase is a hydrophobic phase which is immiscible with the hydrophilic phase. Enzyme molecules are located at or within interfacial boundaries of the droplets and the second liquid phase. The enzyme molecules of the respective droplets are cross-linked so that individual enzyme structures, which are stable and in which the enzymes are immobilized with a majority of active sites of the enzymes being orientated either internally or externally, are formed from individual droplets.
6 Citations
31 Claims
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1. A process for producing enzyme particles, which process comprising includes
providing an emulsion of droplets of a first liquid phase dispersed in a second liquid phase, with the one liquid phase being a hydrophilic phase and the other liquid phase being a hydrophobic phase which is immiscible with the hydrophilic phase, and with enzyme molecules being located at or within interfacial boundaries of the droplets and the second liquid phase; -
cross-linking the enzyme molecules of the respective droplets so that individual enzyme particles, which are stable and in which the enzymes are immobilized with a majority of active sites of the enzymes being orientated either internally or externally, are formed from individual droplets; and recovering the individual enzyme particles from the second liquid phase. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 30, 31)
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- 24. An enzyme particle, which comprises cross-linked enzyme molecules so that the particle is stable, with the particle being hollow, and in which the enzymes are immobilized, with a majority of active sites of the enzymes being orientated either internally or externally.
Specification