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Rapid Method To Detect Nucleic Acid Molecules

  • US 20080220979A1
  • Filed: 08/27/2003
  • Published: 09/11/2008
  • Est. Priority Date: 08/13/2003
  • Status: Abandoned Application
First Claim
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1. A method for detecting a target nucleic acid molecule, said method comprises:

  • a) preparing a cell lysate comprising lysing a cell in a biological sample in a lysis buffer to release the target nucleic acid molecule from the cell;

    b) incubating the cell lysate from step a), without nucleic acid purification, with a nucleic acid probe immobilized on a solid substrate under conditions that allow hybridization between the target nucleic acid molecule and the probe, wherein the nucleic acid probe comprises a sequence complementary to the target nucleic acid molecule;

    c) assessing hybridization between the target nucleic acid molecule and the probe to determine the presence, absence and/or amount of the target nucleic acid molecule.

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