Rapid Method To Detect Nucleic Acid Molecules
First Claim
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1. A method for detecting a target nucleic acid molecule, said method comprises:
- a) preparing a cell lysate comprising lysing a cell in a biological sample in a lysis buffer to release the target nucleic acid molecule from the cell;
b) incubating the cell lysate from step a), without nucleic acid purification, with a nucleic acid probe immobilized on a solid substrate under conditions that allow hybridization between the target nucleic acid molecule and the probe, wherein the nucleic acid probe comprises a sequence complementary to the target nucleic acid molecule;
c) assessing hybridization between the target nucleic acid molecule and the probe to determine the presence, absence and/or amount of the target nucleic acid molecule.
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Abstract
This invention relates to the field of detecting nucleic acid molecules using microarrays. The invention provides a method for detecting a target nucleic acid molecule in a biological sample by hybridizing a cell lysate directly probes immobilized on microarrays without any nucleic acid purification.
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22 Claims
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1. A method for detecting a target nucleic acid molecule, said method comprises:
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a) preparing a cell lysate comprising lysing a cell in a biological sample in a lysis buffer to release the target nucleic acid molecule from the cell; b) incubating the cell lysate from step a), without nucleic acid purification, with a nucleic acid probe immobilized on a solid substrate under conditions that allow hybridization between the target nucleic acid molecule and the probe, wherein the nucleic acid probe comprises a sequence complementary to the target nucleic acid molecule; c) assessing hybridization between the target nucleic acid molecule and the probe to determine the presence, absence and/or amount of the target nucleic acid molecule. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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