PROTEIN PURIFICATION METHOD
First Claim
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1. A method for removing contaminant DNA in a sample containing a physiologically active protein, which comprises the following steps:
- 1) converting the sample containing a physiologically active protein into an acidic or alkaline aqueous solution of low conductivity having a molarity of 100 μ
M or less;
2) adjusting the pH of the resulting sample to pH of 4 to 8 to form particles, wherein the molarity of the adjusted sample is 100 mM or less; and
3) removing the particles thereby to remove contaminant DNA in the sample.
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Abstract
A method for removing contaminant DNA in a sample containing a physiologically active protein, which comprises the following steps:
- 1) converting the sample containing a physiologically active protein into a neutral aqueous solution of low conductivity; and
- 2) removing the resulting particles.
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14 Claims
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1. A method for removing contaminant DNA in a sample containing a physiologically active protein, which comprises the following steps:
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1) converting the sample containing a physiologically active protein into an acidic or alkaline aqueous solution of low conductivity having a molarity of 100 μ
M or less;2) adjusting the pH of the resulting sample to pH of 4 to 8 to form particles, wherein the molarity of the adjusted sample is 100 mM or less; and 3) removing the particles thereby to remove contaminant DNA in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification