Analytical Apparatus with Array of Sensors and Calibrating Element

• US 20080264170A1
• Filed: 06/09/2005
• Published: 10/30/2008
• Est. Priority Date: 06/12/2004
• Status: Active Grant

• Current Assignee: Inverness Medical Switzerland GmbH (Abbott Laboratories Incorporated)
• Original Assignee: Inverness Medical Switzerland GmbH (Abbott Laboratories Incorporated)
• Inventors: Abbott, Julian Paul
• Granted Patent: US 7,878,064 B2
• Time in Patent Office: Days
• US Class Current: 73/590
• CPC Class Codes: G01N 2291/0256 Adsorption, desorption, surface mass change, e.g. on biosensors
• CPC Class Codes: G01N 2291/0426 Bulk waves, e.g. quartz crystal microbalance, torsional waves
• CPC Class Codes: G01N 2291/0427 Flexural waves, plate waves, e.g. Lamb waves, tuning fork, cantilever
• CPC Class Codes: G01N 2291/106 one or more transducer arrays
• CPC Class Codes: G01N 29/022 Fluid sensors based on microsensors, e.g. quartz crystal-microbalance [QCM], surface acoustic wave [SAW] devices, tuning forks, cantilevers, flexural plate wave [FPW] devices microdevices per se B81B
• CPC Class Codes: G01N 29/036 by measuring frequency or resonance of acoustic waves
• CPC Class Codes: G01N 29/30 Arrangements for calibrating or comparing, e.g. with standard objects
• CPC Class Codes: G01N 29/4436 with a reference signal amplitude comparison G01N29/48

Abstract

Analytical apparatus for analysing at least one substance has an array of sensors (1) driven by an electronic driver (14) for operating each sensor. The signals produced by the sensors are received by a receiver (82), and the apparatus includes one or more calibration elements connected to the driver and the receiver for enabling changes in parasitic losses in the apparatus to be determined. The invention is particularly applicable to apparatus in which the sensors are electrical-mechanical transducers, individually connectable (in sequence) to the driver and receiver by a corresponding array of switches forming part of an interface (4).

Claims

1. Analytical apparatus for analysing at least one substance, the apparatus comprising an array of sensors, electronic drive means for operating each sensor, and a receiver for receiving and processing signals from the sensors, wherein the apparatus includes one or more calibration elements, also connected to the drive means and the receiver, for enabling changes in parasitic losses in the apparatus to be determined.

2. Apparatus according to claim 1, in which the calibration elements are such as to enable the parasitic losses, and hence any changes therein, to be determined.

3. Apparatus according to claim 1, in which each sensor comprises a respective electrical-mechanical transducer having an active surface which is oscillated by the signal from the drive means.

4. Apparatus according to claim 3, in which each active surface carries a coating to which the one or more substances can be bound.

5. Apparatus according to claim 4, in which each coating comprises a receptor specific for the one or more substances.

6. Apparatus according to claim 3, in which the transducer comprises a piezoelectric piezo-magnetic or acoustic transducer.

7. Apparatus according to claim 3, in which, the sensor comprises a micro-electromechanical device, such as a membrane, a cantilever, a tuning fork, or other vibrating structure.

8. Apparatus according to claim 3, in which each transducer comprises a quartz crystal resonator.

9. Apparatus according to claim 3, in which the sensor has two electrodes, one of the electrodes constituting the active surface and being earthed.

10. Apparatus according to claim 1, in which the drive means comprises a common driver operable to provide power to the sensors and the calibration elements.

11. Apparatus according to claim 10, in which the apparatus additionally comprises a plurality of switches for enabling each sensor and each calibration element to be individually addressed by the driver and receiver.

12. Apparatus according to claim 11, in which the driver and receiver are connected to the sensors and the calibration elements via a common interface.

13. Apparatus according to claim 11, in which each element and each sensor is connected by the driver by a respective switch.

14. Apparatus according to claim 1, in which the apparatus includes at least three calibration elements.

15. Apparatus according to claim 14, in which all three calibration elements are passive circuit elements.

16. Apparatus according to claim 15, in which a first of the calibration elements comprises a conductive path which provides a short circuit from the switch to earth, the second calibration element comprises an open circuit, and the third of the calibration elements comprises a resistive load of a known finite resistance.

17. Apparatus according to claim 16, in which the second calibration element comprises a gap in a conductive track from the respective switch to earth, the track corresponding to conductive tracks connecting the sensors and the other calibration elements between the switches and earth, and therefore generating parasitic losses corresponding to those associated with the interface with the other calibration elements and the sensors.

18. Apparatus according to claim 1, in which the calibration elements are situated at electrically analogous positions to the sensors.

19. Apparatus according to claim 18, in which the conductive paths from the driver to the calibration elements and the sensors are preferably of substantially the same length as each other.

20. Apparatus according to claim 19, in which the conductive paths from each calibration element and each sensor to earth are also substantially the same length as each other.

21. Apparatus according to claim 1, in which the drive means is

operable to drive each sensor at frequencies distributed over a range of possible frequencies, the receiver being operable to monitor a characteristic of the sensor over said range.

22. Apparatus according to claim 21, in which the drive means, in use, progressively varies said frequency over said range.

23. Apparatus according to claim 20, in which the drive means is operable to cause the oscillation frequency of each sensor to be swept through the resonance frequency of the sensor the receiver being operable to monitor the admittance of the sensor over that range.

24. Apparatus according to claim 21, wherein the sensors have associated differing ranges of frequencies which include the resonance frequencies, or a given overtone thereof, for the sensor when not analysing said substance, the apparatus being so arranged that, during the calibration, the apparatus measures the impedances of the calibration elements at frequencies distributed over a calibration range from the collective minimum to the collective maximum of the ranges associated with the resonance frequencies of the overtone frequencies of sensors.

25. Apparatus according to claim 24, in which, if there is no impedance reading for the calibration elements at a frequency at which a sensor is to be driven, the apparatus is operable to interpolate the impedance readings for the calibration elements to obtain calibration data for that frequency.

26. Apparatus according to claim 25, in which the apparatus is operable to obtain the same number of points of calibration data, over said calibration range, from each calibration element as points of impedance data for each sensor over the respective range for that sensor.

27. Apparatus according to claim 21, in which the apparatus includes a signal processor wherein the signal processor is so arranged that prior to the analysis of the substance to be analysed by the sensor, the signal causes the drive means and the receiver to determine, for each sensor, a respective range of frequencies which includes the resonance frequency, or a range which includes an overtone of the resonance frequency for that sensor, the signal processor also being arranged subsequently to cause each sensor to be driven at frequencies distributed over that range in the course of said analysis.

28. Apparatus according to claim 11, in which the switches are formed as a switching circuit in physical contact with or integrated into the array.

29. Apparatus according to claim 11, in which the array of sensors is composed of a number of groups of sensors, each group being in a respective region of the array and having a respective one or more calibration elements, wherein the switches comprise one or more primary switches for selecting a group and a plurality of secondary switches for selecting a sensor or calibration element from the selected group.

30. Apparatus according to claim 29, in which each calibration element is located in the same region as the group of sensors with which it is associated

31. Apparatus according to claim 1, in which the drive means and receiver are separate components of the apparatus.

36. Apparatus according to claim 3, in which the apparatus is operable to measure overtone frequencies of the sensor.

32. A method of analysing one or more substances, the method comprising the steps of bringing the or each substance into contact with one or more sensors in an array of sensors, supplying electrical power to the sensors and receiving and analysing the signals received from the sensors, wherein the method further comprises the steps of periodically interrogating one or more calibration elements and analysing the signals received from said one or more calibration elements to provide data on at least the changes in parasitic losses in the circuitry connected to the sensors and using said data to compensate for the effects of said changes on the received output from the sensors.

33. A method according to claim 32, in which power is supplied to each sensor in turn in one cycle of operation and at least one calibration element is interrogated once each cycle.

34. A method according to claim 33, in which if the interrogation of said calibration element indicates a significant change in parasitic loss, one or more further different calibration elements are interrogated.

35. A method according to claim 34, in which the further calibration elements are interrogated in the same cycle as the first said calibration element.