Fluorescence-based lateral flow device with improved sensitivity
First Claim
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1. A lateral flow device for performing biological assay comprising:
- (a) a chromatographic strip consisting of a support membrane pad, wherein a sample application pad, a fluorescent-labeled conjugate release pad, and an absorbent pad are positioned in series on a top surface of said support pad;
(b) fluorescence nanoparticles embedded within said conjugate release pad; and
(c) an analyte entering site adjacent said sample application pad.
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Abstract
The invention describes how to use nanometer scale fluorescence particles as a label material for fluorescence lateral flow device application. The utilization of the nanoparticles instantly increases the fluorescence intensity by thousands to millions of times. The resulting signal enhancement not only significantly increase sensitivity for analyte detection, but also makes it possible to use low power light sources for illumination and low cost detectors for fluorescence detection.
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18 Claims
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1. A lateral flow device for performing biological assay comprising:
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(a) a chromatographic strip consisting of a support membrane pad, wherein a sample application pad, a fluorescent-labeled conjugate release pad, and an absorbent pad are positioned in series on a top surface of said support pad; (b) fluorescence nanoparticles embedded within said conjugate release pad; and (c) an analyte entering site adjacent said sample application pad. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification