NUCLEIC ACID DETECTION COMBINING AMPLIFICATION WITH FRAGMENTATION

  • US 20090181378A1
  • Filed: 02/21/2008
  • Published: 07/16/2009
  • Est. Priority Date: 04/27/2007
  • Status: Active Grant
First Claim
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1. A method for detecting the presence or absence of a target nucleic acid by testing a sample that potentially contains the target nucleic acid in the presence of non-target nucleic acid, said method comprising:

  • a) fragmenting the sample nucleic acid under conditions such that a subsequent amplification directed to the target nucleic acid results in an increased detection of said target nucleic acid over said non-target nucleic acid as compared to amplification without fragmentation;

    b) amplifying the target nucleic acid with a pair of primers, wherein a first primer is specific for the target nucleic acid; and

    c) detecting the presence or absence of an amplification product, which indicates the presence or absence of the target nucleic acid in the sample.

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