MICROWAVE-ASSISTED EXTRACTION OF POLYSACCHARIDE FROM ARTEMISIA SPHAEROCEPHALA
First Claim
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1. A process for producing crude polysaccharide from Artemisia sphaerocephala with microwave-assistance comprising:
- a. Artemisia sphaerocephala seed powder was obtained by CO2 supercritical fluid extraction of oil. The said powder was mixed with distilled water. The ratio of the mixture was 1;
30-1;
50 (w/w). The suspension was irradiated with microwaves in microwave extraction equipment with water condenser. The overall microwave power was 600 W-800 W with the frequency of 915 MHz for 20-30 min at the temperature of 70-80°
C. The extraction product may be recovered from the extractant after separation from the solids material as filtering at the pressure of 0.07-0.09 MPa. Distilled water was evaporated off from the resulting solvent extract at the pressure of 0.06-0.08 MPa, and the total volume of the extraction was reduced to ⅛
-¼
of original. Ethanol was then added in concentrated extract (ethanol content was about 70%-80% (v/v) in total extract). The precipitate in ethanol solution was then collected by centrifugation at the centrifugal speed of 3500-4000 rpm after stored in static condition for 20-30 h. The said collected precipitate was freeze-drying for 24-36 h at the temperature of −
60-−
50°
C. with the pressure of 1 Pa, get material A.b. Material A was dissolved in distilled water, the ratio of the mixture was 1;
150-1;
200 (w/w). The aqueous solution of extract was incubated with 6 u-8 u/100 ml papain for 10-12 h after heated at the temperature of 60-70°
C. The said extract was purified by butanol/chloroform (1;
2-1;
4, v/v) for 3-6 times. The solution in aqueous phase was collected and concentrated to ⅛
-¼
of original volume at the temperature of 40-50°
C., 0.06-0.08 Mpa, get material B.c. The pH value of material B was adjusted to 9 by adding ammonia. The solution of extract was decolorizated by dropping 20%-30% H2O2 until the color of the said solution turned to colorless. The said colorless solution was concentrated to 1/80-¼
of original volume at the pressure of 0.06-0.08 Mpa. The concentrated solution was then dialyzed (Mw cut off 8000-14000 Da) against distilled water for 36-48 h. Ethanol was added in extract solution (ethanol content was about 70%-80% (v/v) in total extract) after dialysis. The precipitate in ethanol solution was then collected by centrifugation at the centrifugal speed of 3500-4000 rpm after stored in static condition for 10-14 h. The said collected precipitate was freeze-drying for 24-36 h at the temperature of −
60-−
50°
C. with the pressure of 1 Pa, get purified ASP.
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Abstract
This invention relates to a method of extracting crude polysaccharide by microwave-assisted from Artemisia sphaerocephala seed de-oiling dregs. In particular, the invention comprising: microwave-assisted extraction, enzymatic hydrolysis, dialysis et. al. It provides a technique whereby the polysaccharide can be extracted effectively, in a relatively short period of time (20-30 min) with respect to conventional extraction methods and allows for an enhanced extraction yield (more than 30%). Furthermore, the invention also allows for the extraction of material with less solvent consumption, good reproducibility, excellent solubility, energy conservation, environmental protection, and showed great potential for efficient sample preparation and large-scale industrial application in the near future. With great resource advantage of Artemisia sphaerocephala in China, the invention has bright perspectives in promoting local economic development.
3 Citations
1 Claim
-
1. A process for producing crude polysaccharide from Artemisia sphaerocephala with microwave-assistance comprising:
-
a. Artemisia sphaerocephala seed powder was obtained by CO2 supercritical fluid extraction of oil. The said powder was mixed with distilled water. The ratio of the mixture was 1;
30-1;
50 (w/w). The suspension was irradiated with microwaves in microwave extraction equipment with water condenser. The overall microwave power was 600 W-800 W with the frequency of 915 MHz for 20-30 min at the temperature of 70-80°
C. The extraction product may be recovered from the extractant after separation from the solids material as filtering at the pressure of 0.07-0.09 MPa. Distilled water was evaporated off from the resulting solvent extract at the pressure of 0.06-0.08 MPa, and the total volume of the extraction was reduced to ⅛
-¼
of original. Ethanol was then added in concentrated extract (ethanol content was about 70%-80% (v/v) in total extract). The precipitate in ethanol solution was then collected by centrifugation at the centrifugal speed of 3500-4000 rpm after stored in static condition for 20-30 h. The said collected precipitate was freeze-drying for 24-36 h at the temperature of −
60-−
50°
C. with the pressure of 1 Pa, get material A.b. Material A was dissolved in distilled water, the ratio of the mixture was 1;
150-1;
200 (w/w). The aqueous solution of extract was incubated with 6 u-8 u/100 ml papain for 10-12 h after heated at the temperature of 60-70°
C. The said extract was purified by butanol/chloroform (1;
2-1;
4, v/v) for 3-6 times. The solution in aqueous phase was collected and concentrated to ⅛
-¼
of original volume at the temperature of 40-50°
C., 0.06-0.08 Mpa, get material B.c. The pH value of material B was adjusted to 9 by adding ammonia. The solution of extract was decolorizated by dropping 20%-30% H2O2 until the color of the said solution turned to colorless. The said colorless solution was concentrated to 1/80-¼
of original volume at the pressure of 0.06-0.08 Mpa. The concentrated solution was then dialyzed (Mw cut off 8000-14000 Da) against distilled water for 36-48 h. Ethanol was added in extract solution (ethanol content was about 70%-80% (v/v) in total extract) after dialysis. The precipitate in ethanol solution was then collected by centrifugation at the centrifugal speed of 3500-4000 rpm after stored in static condition for 10-14 h. The said collected precipitate was freeze-drying for 24-36 h at the temperature of −
60-−
50°
C. with the pressure of 1 Pa, get purified ASP.
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Current AssigneeNorthwest Normal University
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Original AssigneeNorthwest Normal University
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InventorsWANG, Xiaodong, ZHANG, Yanan, WU, Yiqian, XU, Xiaoqing, ZHANG, Ji, YAO, Jian, LIANG, Junyu, WANG, Yunpu, WANG, Junlong
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Granted Patent
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Time in Patent OfficeDays
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Field of Search
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US Class Current536/128
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CPC Class CodesC08B 37/0003 General processes for their...C08B 37/006 Heteroglycans, i.e. polysac...
