DIAGNOSTIC TEST FOR VITAMIN B12

• US 20090305348A1
• Filed: 07/08/2009
• Published: 12/10/2009
• Est. Priority Date: 02/07/2005
• Status: Active Grant

• Current Assignee: Abbott Laboratories Incorporated
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Wonderling, Ramani S., Uher, John F.
• Granted Patent: US 7,932,369 B2
• Time in Patent Office: Days
• US Class Current: 435/69.100
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: G01N 33/82 involving vitamins or their receptors
• CPC Class Codes: Y10T 436/11 Automated chemical analysis

Abstract

An isolated nucleotide sequence or fragment thereof encoding the porcine intrinsic factor, wherein the porcine intrinsic factor comprises an amino acid sequence having at least 85% amino acid sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 6, and SEQ ID NO: 9. The invention also encompasses an isolated nucleic acid sequence or fragment thereof comprising, or complementary to, a nucleotide sequence having at least 85% nucleotide sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 4, and SEQ ID NO: 7. The porcine intrinsic factor can be use is an assay to determine the quantity of vitamin B₁₂ in a biological sample.

Claims

1. An isolated nucleic acid sequence or fragment thereof comprising or complementary to a nucleotide sequence encoding porcine intrinsic factor, wherein the amino acid sequence of said porcine intrinsic factor has at least 85% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:

3, SEQ ID NO;

6, and SEQ ID NO;

9.

2. The nucleotide sequence of claim 1 wherein said sequence is isolated from Sus genus.

3. The nucleotide sequence of claim 2 wherein said species of said Sus genus is Sus scrofa.

4. An isolated nucleic acid sequence or fragment thereof comprising or complementary to a nucleotide sequence having at least 85% identity to a nucleotide sequence selected from the group consisting of SEQ ID NO:

1, SEQ ID NO;

4, and SEQ ID NO;

7.

5. The nucleotide sequence of claim 4 wherein said sequence is isolated from Sus genus.

6. The nucleotide sequence of claim 5 wherein said species of said Sus genus is Sus scrofa.

7. A method of producing porcine intrinsic factor comprising the steps of:

(a) isolating a nucleic acid sequence comprising or complementary to a nucleotide sequence;

i) encoding porcine intrinsic factor comprising an amino acid sequence having at least 85% identity to an amino acid sequence selected from the group consisting of SEQ ID NO;

3, SEQ ID NO;

6, and SEQ ID NO;

9, or

ii) having at least 85% identity to a nucleotide sequence selected from the group consisting of SEQ ID NO;

1, SEQ ID NO;

4, and SEQ ID NO;

7;

(b) constructing a vector comprising;

i) said isolated nucleotide sequence operably linked to ii) a regulatory sequence;

(c) introducing said vector into a host cell for a time and under conditions sufficient for expression of said porcine intrinsic factor.

8. A vector comprising:

(a) an isolated nucleic acid sequence comprising or complementary to a nucleotide sequence;

i) encoding a polypeptide comprising an amino acid sequence having at least 85% identity to an amino acid sequence selected from the group consisting of SEQ ID NO;

3, SEQ ID NO;

6, and SEQ ID NO;

9, or

ii) having at least 85% identity to a nucleotide sequence selected from the group consisting of SEQ ID NO;

1, SEQ ID NO;

4, and SEQ ID NO;

7, operably linked to

(b) a regulatory sequence.

9. An isolated nucleic acid sequence or fragment thereof which hybridizes, under moderate or high stringency conditions, to a nucleic acid sequence selected from the group consisting of SEQ ID NO:

1, SEQ ID NO;

4, and SEQ ID NO;

7.

10. An isolated nucleic acid or fragment thereof, which hybridizes, under moderate or high stringency conditions, to an isolated nucleic acid sequence encoding porcine intrinsic factor, wherein the amino acid sequence of said polypeptide has at least 85% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:

3, SEQ ID NO;

6, and SEQ ID NO;

9.