Transformation of Algal Cells
First Claim
1. A method for transforming an algal cell, the method comprising:
- preparing a transformation construct;
preparing a particle for bombarding the algal cell;
adhering the transformation construct to the particle;
bombarding the algal cell with the particle; and
growing the algal cell into a colony.
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Abstract
Exemplary methods include a method for transforming an algal cell by preparing a transformation construct, preparing a particle for bombarding the algal cell, adhering the transformation construct to the particle, bombarding the algal cell with the particle, and growing the algal cell into a colony. The transformation construct is replicated within a nuclear genome of the algal cell and the growing of the algal cell is in a nutrient medium. Another exemplary method may include a method for genetically modifying an algal cell, by adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity, introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell, and selecting a colony that includes the transformed algal cell.
83 Citations
23 Claims
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1. A method for transforming an algal cell, the method comprising:
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preparing a transformation construct; preparing a particle for bombarding the algal cell; adhering the transformation construct to the particle; bombarding the algal cell with the particle; and growing the algal cell into a colony. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 22)
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16. A method for genetically modifying an algal cell, the method comprising:
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adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity; and introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell; and selecting a colony that includes the transformed algal cell. - View Dependent Claims (17, 18, 19, 20, 21, 23)
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Specification