USING POPULATIONS OF BEADS FOR THE FABRICATION OF ARRAYS ON SURFACES
First Claim
1. A method for fabricating an array of nucleic acids on a surface comprising:
- (a) providing a surface comprising one or more primer oligonucleotides attached to the surface;
(b) providing a pool of beads, wherein beads in the pool have a plurality of templates attached thereto, the plurality comprising multiple copies of a single nucleic acid template sequence;
(c) arraying the beads onto the surface by hybridizing the templates to the primer oligonucleotides; and
(d) extending the primers to produce copies of the templates attached to the surface.
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Accused Products
Abstract
The present invention provides methods for creating an array of features on a surface based on content transferred from a plurality of beads to the surface. Nucleic acid content can be transferred using a method including the steps of (a) providing a surface having one or more primer oligonucleotides attached to the surface; (b) providing a pool of beads, wherein beads in the pool have a plurality of templates attached thereto, the plurality comprising multiple copies of a single nucleic acid template sequence; (c) arraying the beads onto the surface by hybridizing the templates to the primer oligonucleotides; and (d) extending the primers to produce copies of the templates attached to the surface.
63 Citations
18 Claims
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1. A method for fabricating an array of nucleic acids on a surface comprising:
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(a) providing a surface comprising one or more primer oligonucleotides attached to the surface; (b) providing a pool of beads, wherein beads in the pool have a plurality of templates attached thereto, the plurality comprising multiple copies of a single nucleic acid template sequence; (c) arraying the beads onto the surface by hybridizing the templates to the primer oligonucleotides; and (d) extending the primers to produce copies of the templates attached to the surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A composition comprising
(a) an array of beads on a surface, wherein the beads are attached to template nucleic acids, wherein the surface is attached to oligonucleotide primers and wherein the beads are immobilized on the surface via hybridization of the template nucleic acids to one or more of the oligonucleotide primers; - and
(b) a nucleic acid polymerase that is bound to hybrids formed between the template nucleic acids and the oligonucleotide primers.
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Specification