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HIGH SENSITIVITY DETERMINATION OF THE CONCENTRATION OF ANALYTE MOLECULES OR PARTICLES IN A FLUID SAMPLE

  • US 20100075862A1
  • Filed: 09/23/2008
  • Published: 03/25/2010
  • Est. Priority Date: 09/23/2008
  • Status: Abandoned Application
First Claim
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1. A method of detecting analyte molecules or particles in a fluid sample containing or suspected of containing analyte molecules or particles, comprising:

  • partitioning the fluid sample across an array comprising a plurality of reaction vessels, so that at least some of the reaction vessels contain no analyte molecules or particles and at least some of the reaction vessels contain at least one analyte molecule or particle;

    immobilizing at least one binding ligand with respect to an analyte molecule or particle within each reaction vessel containing at least one analyte molecule or particle;

    exposing the at least one binding ligand to a liquid in which is solubilized or suspended precursor labeling agent molecules, wherein exposure of the precursor labeling agent molecules to the at least one binding ligand converts at least some of the precursor labeling agent molecules into a labeling agent molecules which are insoluble in the liquid and/or which become immobilized within the reaction vessel; and

    determining from detecting the presence of the labeling agent molecules the number of reaction vessels which contain an analyte molecule or particle.

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