System and Method For the Separation of Analytes
First Claim
1. A process for separating a sample comprising:
- introducing a sample into a first microchannel formed in a module;
separating the sample into a plurality of sub-samples based on the varying isoelectric points of the plurality of sub-samples;
causing a first of the plurality of sub-samples to enter a first of a plurality of second parallel microchannels integrally connected to the first microchannel; and
separating the first of the plurality of sub-samples into a plurality of protein components according to electrophoresis.
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Accused Products
Abstract
A separation module operates to fractionate or separate an analyte into fractions according to pI, i.e., pI bands, utilizing capillary isoelectric focusing (“CIEF”) within a first microchannel. The fractions are stacked to form plugs, the number of which is determined by a number of parallel second microchannels integrally connected to the first microchannel, into which the fractions are directed according to the buffer characteristics found in each of the individual microchannels. Within the microchannels the plugs are separated into proteins according to a different chemical property, i.e., “m/z,” utilizing capillary electrophoresis (“CE”).
44 Citations
8 Claims
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1. A process for separating a sample comprising:
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introducing a sample into a first microchannel formed in a module; separating the sample into a plurality of sub-samples based on the varying isoelectric points of the plurality of sub-samples; causing a first of the plurality of sub-samples to enter a first of a plurality of second parallel microchannels integrally connected to the first microchannel; and separating the first of the plurality of sub-samples into a plurality of protein components according to electrophoresis. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification