DNA LIGATION ON RNA TEMPLATE

• US 20100184618A1
• Filed: 01/19/2010
• Published: 07/22/2010
• Est. Priority Date: 01/16/2009
• Status: Active Grant

• Current Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: MIAO, XIN, BLUME, JOHN E., NAMSARAEV, EUGENI A.
• Granted Patent: US 8,790,873 B2
• Time in Patent Office: Days
• US Class Current: 506/9
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2533/107 Probe or oligonucleotide ligation
• CPC Class Codes: C12Q 2537/143 Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
• CPC Class Codes: C12Q 2537/162 Helper probe

Abstract

Disclosed are methods and compositions for detection and amplification of nucleic acids, wherein two DNA strands hybridized to an RNA strand are ligated. In one aspect, the disclosed methods include removal of an energy source, such as ATP, upon charging a ligase to form an enzyme-AMP intermediate, and then adding substrate, which results in one complete round of RNA-templated DNA ligation. In another aspect, the ligation reaction is accomplished by use of a mixture of at least two different ligase enzymes. The disclosed methods and compositions for RNA-templated DNA ligation may be particularly useful for detection of RNA sequence variants, for example RNA splice variants, and for quantitative expression analysis.

Claims

1. A method for detecting a plurality of target nucleic acids from a nucleic acid sample, comprising:

a) contacting a DNA array-bound probe with;

(i) a DNA interrogation probe, and

(ii) a nucleic acid sample comprising at least one RNA target nucleic acid,

wherein the array-bound probe and the interrogation probe hybridize to the target nucleic acid such that the 3′

end of the array-bound probe and the 5′

end of the interrogation probe are directly adjacent to one another and ligatable by DNA ligase, and wherein the interrogation probe contains a detectable label;

b) adding an effective amount of ATP to a solution of DNA ligase, thereby charging the DNA ligase to form a DNA ligase-AMP intermediate,

c) depleting ATP from the solution of DNA ligase-AMP intermediate;

d) adding the solution of DNA ligase-AMP intermediate to step a), thereby ligating the 3′

end of the array-bound probe to the 5′

end of the interrogation probe; and

e) detecting the ligated product.

2. The method according to claim 1, wherein ATP depletion is accomplished by adding an effective amount of apyrase in conditions which allow apyrase to catalytically convert ATP in solution to ADP and/or AMP.

3. The method according to claim 1, wherein ATP depletion is accomplished by adding an effective amount of hexokinase in conditions which allow hexokinase to catalytically convert ATP in solution to ADP and/or AMP.

4. The method according to claim 1, wherein the DNA ligase is T4 DNA ligase.

5. The method according to claim 2, wherein there is about 10 mU apyrase per μ

l of ATP depletion mix.

6. The method according to claim 3, wherein there is about 0.4 U hexokinase per μ

l of ATP depletion mix.

7. The method of claim 1 wherein the concentration of ATP in the ligase charging mix is between 200 and 500 mM.

8. A method for detecting a plurality of target nucleic acids from a nucleic acid sample, comprising:

a) contacting a DNA array-bound probe with;

(i) a DNA interrogation probe, and

(ii) an RNA nucleic acid sample comprising at least one target nucleic acid,

wherein the array-bound probe and the interrogation probe hybridize to the target nucleic acid such that the 3′

end of the array-bound probe and the 5′

end of the interrogation probe are directly adjacent to one another and ligatable by DNA ligase, and wherein the interrogation probe contains a detectable label;

b) adding an effective amount of ATP to a solution of at least two different DNA ligase enzymes, thereby charging the at least one of the at least two different DNA ligase enzymes to form a DNA ligase-AMP intermediate complex,

wherein the at least two different DNA ligase enzymes comprise at least a first ligase which when charged to form a DNA ligase-AMP intermediate complex possesses activity to form a 5′

to 5′

pyrophosphate bond on the interrogation probe 5′

terminus, and at least a second ligase which is a mutant ligase unable to form a DNA ligase-AMP intermediate complex, but which possesses an activity of forming a covalent phosphodiester bond from the 5′

to 5′

pyrophosphate bond of the interrogation probe and the 3′

hydroxyl terminus of the array-bound probe;

c) depleting ATP from the solution of at least two different DNA ligase enzymes;

d) adding the solution of at least two different DNA ligase enzymes to step a), thereby ligating the 3′

end of the array-bound probe to the 5′

end of the interrogation probe; and

e) detecting the ligated product.

9. The method according to claim 8, wherein the second ligase is E. coli NAD⁺-dependent ligase LigA which comprises a point mutant or an amino terminal truncation.

10. The method according to claim 8, wherein the second ligase is an E. coli NAD⁺-dependent ligase LigA mutant selected from the group consisting of:

Y22A, D32A and D36A.

11. A method of detecting a plurality of target nucleic acids from a nucleic acid sample, comprising:

a) contacting an open circle DNA probe with an RNA nucleic acid sample comprising at least one target nucleic acid, wherein the open circle probe comprises a 5′

end and a 3′

end, and wherein the open circle probe contains a detectable label;

b) adding an effective amount of ATP to a solution of DNA ligase, thereby charging the DNA ligase to form a DNA ligase-AMP intermediate,

c) depleting ATP from the solution of DNA ligase-AMP intermediate;

d) adding the solution of DNA ligase-AMP intermediate to step a), thereby ligating the 5′

end of the open circle probe to the 3′

end of the open circle probe; and

e) detecting the ligated product.

12. The method according to claim 11, wherein ATP depletion is accomplished by adding an effective amount of apyrase in conditions which allow apyrase to catalytically convert ATP in solution to ADP and/or AMP.

13. The method according to claim 11, wherein ATP depletion is accomplished by adding an effective amount of hexokinase in conditions which allow hexokinase to catalytically convert ATP in solution to ADP and/or AMP.

14. The method according to claim 11, wherein the DNA ligase is T4 DNA ligase.

15. The method according to claim 12, wherein the effective amount of apyrase is about 10 mU apyrase per μ

l.

16. The method according to claim 13, wherein the effective amount of hexokinase is about 0.4 U hexokinase per μ

l.

17. The method of claim 11 wherein the concentration of ATP in the ligase charging mix is between 200 and 500 mM.

18. The method of claim 11 wherein the concentration of ATP in the ligase charging mix is between 100 and 1000 mM.

19. The method of claim 11 wherein the concentration of ATP in the ligase charging mix is between 0.1 and 200 mM.

20. The method of claim 11 wherein the concentration of ATP in the ligase charging mix is between 10 and 100 mM.