Digital Amplification

  • US 20100209921A1
  • Filed: 11/12/2009
  • Published: 08/19/2010
  • Est. Priority Date: 08/02/1999
  • Status: Active Grant
First Claim
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1. A method for determining an allelic imbalance in a biological sample, comprising the steps of:

  • amplifying template molecules within a set comprising a plurality of assay samples to form a population of amplified molecules in each of the assay samples of the set, wherein the template molecules are obtained from the biological sample;

    analyzing the amplified molecules in the assay samples of the set to determine a first number of assay samples which contain a first allelic form of a marker and a second number of assay samples which contain a second allelic form of the marker, wherein between 0.1 and 0.9 of the assay samples yield an amplification product;

    comparing the first number to the second number to ascertain an allelic imbalance in the biological sample; and

    identifying an allelic imbalance in the biological sample.

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