L-AMINO ACID-PRODUCING BACTERIUM AND A METHOD FOR PRODUCING AN L-AMINO ACID
First Claim
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1. A method for producing an L-amino acid comprising:
- a) culturing in a medium a bacterium belonging to the family Enterobacteriaceae, which is able to produce an L-amino acid, and has been modified to have a mutation in a yeaS gene selected from the group consisting of;
(I) replacing the threonine residue at position 28 with an amino acid residue other than threonine in the protein encoded by the yeaS gene,(II) replacing the phenylalanine residue at position 137 with an amino acid residue other than phenylalanine residue in the protein encoded by the yeaS gene,(III) replacing the leucine residue at position 188 with an amino acid residue other than leucine in the protein encoded by the yeaS gene, and,(IV) combinations thereof; and
b) collecting the L-amino acid from the medium;
wherein the yeaS gene without said mutation encodes a protein selected from the group consisting of;
(A) a protein comprising the amino acid sequence of SEQ ID NO;
2, and(B) a protein comprising the amino acid sequence of SEQ ID NO;
2, but wherein 1 to 10 amino acid residues are substituted, deleted, inserted or added, and the protein has an activity of improving an L-cysteine-producing ability in the bacterium belonging to the family Enterobacteriaceae as compared to a non-modified bacterium when expression of the protein is increased in the bacterium.
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Abstract
A bacterium belonging to the family Enterobacteriaceae, which has an ability to produce an amino acid such as L-cysteine and has been modified to have specific mutation in the yeas gene, is cultured in a medium, and the L-amino acid is collected from the medium.
24 Citations
15 Claims
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1. A method for producing an L-amino acid comprising:
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a) culturing in a medium a bacterium belonging to the family Enterobacteriaceae, which is able to produce an L-amino acid, and has been modified to have a mutation in a yeaS gene selected from the group consisting of; (I) replacing the threonine residue at position 28 with an amino acid residue other than threonine in the protein encoded by the yeaS gene, (II) replacing the phenylalanine residue at position 137 with an amino acid residue other than phenylalanine residue in the protein encoded by the yeaS gene, (III) replacing the leucine residue at position 188 with an amino acid residue other than leucine in the protein encoded by the yeaS gene, and, (IV) combinations thereof; and b) collecting the L-amino acid from the medium; wherein the yeaS gene without said mutation encodes a protein selected from the group consisting of; (A) a protein comprising the amino acid sequence of SEQ ID NO;
2, and(B) a protein comprising the amino acid sequence of SEQ ID NO;
2, but wherein 1 to 10 amino acid residues are substituted, deleted, inserted or added, and the protein has an activity of improving an L-cysteine-producing ability in the bacterium belonging to the family Enterobacteriaceae as compared to a non-modified bacterium when expression of the protein is increased in the bacterium. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A bacterium belonging to the family Enterobacteriaceae, which is able to produce an L-amino acid, and has been modified to have a mutation in a yeaS gene selected from the group consisting of:
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(I) replacing the threonine residue at position 28 with an amino acid residue other than threonine in the protein encoded by the yeaS gene, (II) replacing the phenylalanine residue at position 137 with an amino acid residue other than phenylalanine in the protein encoded by the yeaS gene, and (III) replacing the leucine residue at position 188 with an amino acid residue other than leucine in the protein encoded by the yeaS gene, and, (IV) combinations thereof; and wherein the yeaS gene without said mutation encodes a protein selected from the group consisting of; (A) a protein comprising the amino acid sequence of SEQ ID NO;
2, and(B) a protein comprising the amino acid sequence of SEQ ID NO;
2, but wherein 1 to 10 amino acid residues are substituted, deleted, inserted or added, and the protein has an activity of improving the ability to produce L-cysteine when overexpressed in a bacterium belonging to the family Enterobacteriaceae as compared to a bacterium in which the protein is not overexpressed. - View Dependent Claims (14)
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15. A DNA which encodes a protein selected from the group consisting of:
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(A) a protein comprising the amino acid sequence of SEQ ID NO;
2, and(B) a protein comprising the amino acid sequence of SEQ ID NO;
2, but wherein 1 to 10 amino acid residues are substituted, deleted, inserted or added, and the protein has an activity of improving the ability to produce L-cysteine when overexpressed in a bacterium belonging to the family Enterobacteriaceae as compared to a bacterium in which the protein is not overexpressed,wherein the protein has a mutation selected from the group consisting of; (I) replacing the threonine residue at position 28 with an amino acid residue other than threonine, (II) replacing the phenylalanine residue at position 137 with an amino acid residue other than phenylalanine, (III) replacing the leucine residue at position 188 with an amino acid residue other than leucine.
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Specification