Branched Acetylene-Containing Poly(Alkylene Oxides, Oxyethylated Polyols or Olefinic Alcohols)

• US 20100217050A1
• Filed: 05/06/2010
• Published: 08/26/2010
• Est. Priority Date: 10/09/2003
• Status: Active Grant

• Current Assignee: Ambrx, Inc. (Johnson & Johnson)
• Original Assignee: Ambrx, Inc. (Johnson & Johnson)
• Inventors: WILSON, Troy E.
• Granted Patent: US 7,820,766 B2
• Time in Patent Office: Days
• US Class Current: 568/616
• CPC Class Codes: C08F 216/16 Monomers containing no hetero atoms other than the ether oxygen
• CPC Class Codes: C08F 8/02 Alkylation
• CPC Class Codes: C08F 8/30 Introducing nitrogen atoms or nitrogen-containing groups
• CPC Class Codes: C08G 65/325 containing nitrogen
• CPC Class Codes: C08G 65/329 with organic compounds
• CPC Class Codes: C08G 65/333 containing nitrogen

Abstract

The invention provides water-soluble compounds that include a polymer and at least one terminal azide or acetylene moiety. Also provided are highly efficient methods for the selective modification of proteins with PEG derivatives, which involves the selective incorporation of non-genetically encoded amino acids, e.g., those amino acids containing an azide or acetylene moiety, into proteins in response to a selector codon and the subsequent modification of those amino acids with a suitably reactive PEG derivative.

Claims

1-47. -47. (canceled)

48. A water-soluble compound comprising a polymer and at least one acetylene moiety, wherein said polymer is selected from the group consisting of poly(alkylene oxides), poly(oxyethylated polyols), and poly(olefinic alcohols), and

wherein said polymer comprises at least one arm of a branched molecular structure.

49. The water-soluble compound of claim 48 wherein said branched molecular structure is dendritic.

50-74. -74. (canceled)