Method for glucose monitoring using fluorescence quenching
First Claim
1. A method of measuring glucose, said method comprising:
- contacting an implantable glucose-sensing device with a test sample under conditions that permit a sugar-binding molecule comprising Concanavalin A, other sugar-binding lectins, glucose binding protein, glucose oxidase, or glucose apo-oxidase, the sugar binding molecule present throughout a matrix of a hydrogel in the glucose-sensing device and a functionalized polymer or functionalized nano-particle ligand present throughout the matrix of the hydrogel in the glucose-sensing device to interact in a glucose dependent manner to produce a glucose-dependent optical signal resulting from quenching of a first fluorophore comprising a fluorescein, a rhodamine, a cyanine, or fluorescent dye, the first fluorophore linked to the ligand or sugar-binding molecule and having a fluorescent emission spectrum quenchable upon binding or release of glucose by the sugar-binding molecule;
exciting the first fluorophore with light of a wavelength sufficient to excite the fluorophore;
detecting at least one wavelength of light in the glucose-dependent optical signal from the fluorophore with a detector to produce a detected light signal; and
processing the detected light signal to produce a glucose metric.
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Accused Products
Abstract
This disclosure relates to systems, devices, and methods of sensing an analyte. An implantable sensor may be contacted with a test sample under conditions that permit a binding protein and a ligand of the sensor to interact in an analyte-dependent manner to produce an analyte-dependent signal, and (b) detecting the analyte-dependent signal with a detector. A binding protein may reversibly bind an analyte and/or a ligand. A binding protein may have a higher binding affinity for an analyte than for a ligand. A binding protein and a ligand may each include a fluorophore, the absorption and/or emission properties of which may change in an analyte-dependent manner. A binding protein and/or a ligand may be bound to an active or inactive substrate. Some embodiments of systems, devices, and methods may be practiced in vitro, in situ, and/or in vivo. Systems and/or devices of the disclosure may be configured to be wearable.
31 Citations
24 Claims
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1. A method of measuring glucose, said method comprising:
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contacting an implantable glucose-sensing device with a test sample under conditions that permit a sugar-binding molecule comprising Concanavalin A, other sugar-binding lectins, glucose binding protein, glucose oxidase, or glucose apo-oxidase, the sugar binding molecule present throughout a matrix of a hydrogel in the glucose-sensing device and a functionalized polymer or functionalized nano-particle ligand present throughout the matrix of the hydrogel in the glucose-sensing device to interact in a glucose dependent manner to produce a glucose-dependent optical signal resulting from quenching of a first fluorophore comprising a fluorescein, a rhodamine, a cyanine, or fluorescent dye, the first fluorophore linked to the ligand or sugar-binding molecule and having a fluorescent emission spectrum quenchable upon binding or release of glucose by the sugar-binding molecule; exciting the first fluorophore with light of a wavelength sufficient to excite the fluorophore; detecting at least one wavelength of light in the glucose-dependent optical signal from the fluorophore with a detector to produce a detected light signal; and processing the detected light signal to produce a glucose metric. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method of measuring glucose in a patient, said method comprising:
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implanting an implantable glucose-sensing device in a patient; contacting an implantable glucose-sensing device with a test sample under conditions that permit a sugar-binding molecule comprising Concanavalin A, other sugar-binding lectins, glucose binding protein, glucose oxidase, or glucose apo-oxidase, the sugar binding molecule present throughout a matrix of a hydrogel in the glucose-sensing device and a functionalized polymer or functionalized nano-particle ligand present throughout the matrix of the hydrogel in the glucose-sensing device to interact in a glucose dependent manner to produce a glucose-dependent optical signal resulting from quenching of a first fluorophore comprising a fluorescein, a rhodamine, a cyanine, or fluorescent dye, the first fluorophore linked to the ligand or sugar-binding molecule and having a fluorescent emission spectrum quenchable upon binding or release of glucose by the sugar-binding molecule; exciting the first fluorophore with light of a wavelength sufficient to excite the fluorophore; detecting at least one wavelength of light in the glucose-dependent optical signal from the fluorophore with a detector to produce a detected light signal; and processing the detected light signal to produce a glucose metric. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification