MEDIA AND PROCESSES FOR THE EX VIVO PRODUCTION OF MEGAKARYOCYTES FROM HUMAN CD34+ CELLS

  • US 20100227401A1
  • Filed: 04/05/2010
  • Published: 09/09/2010
  • Est. Priority Date: 08/06/2003
  • Status: Abandoned Application
First Claim
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1. A process for the ex vivo production of megakaryocytes from human CD34+ cells, comprising:

  • cultivating a population of human CD34+ cells in a cultivating medium consisting essentially of a basal medium, a serum substitute and a cytokine cocktail, wherein;

    the serum substitute consists essentially of human serum albumin, insulin, and transferrin; and

    the cytokine cocktail consists essentially of thrombopoietin, stem cell factor, Flt-3 ligand, interleukin-3, interleukin-6, interleukin-9, and granulocyte-macrophage colony-stimulating factor; and

    harvesting a population of megakaryocytes thus formed from the cultivation of the population of human CD34+ cells.

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