TRANSPLANTATION OF HUMAN NEURAL CELLS FOR TREATMENT OF NEURODEGENERATIVE CONDITIONS
First Claim
1. A method of expanding in vitro at least one neural stem cell to an expanded population of neural stem cells, wherein each neural stem cell expansion exceeds thirty cell doublings without differentiating, said method comprising:
- dissociating neural stem cells from central nervous system tissue;
providing at least one extracellular protein to a culture vessel, wherein the extracellular protein includes at least about 10 μ
g/mL of poly-D-lysine and less than about 1 mg/ml fibronectin;
culturing the dissociated neural stem cells in said culture vessel in the absence of serum;
adding to the culture vessel at least one growth factor; and
passaging the cultured cells prior to confluence.
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Abstract
A method of treating neurodegenerative conditions is provided. Neural stem cells may be implanted at and/or remote from a region of neuron degeneration. The methods can include isolating neural stem cells from regions where specific types of neurons corresponding to the neurons to be replaced are generated. The methods can include isolating neural stem cells secreting growth factors affecting the growth and/or regeneration of specific types of neuron. In this invention, we disclose a method of treating such disorders, including several neurodegenerative disorders arising from the lack of cells that produce particular neurotransmitters in neural circuitry by transplanting exogenously cultured and expanded neural progenitors which, upon transplantation into a neural tissue, differentiate into neurons capable of integrating and producing neurotransmitters in sufficient quantities and in a sufficient manner to overcome the symptoms associated with the neurodegeneration.
44 Citations
10 Claims
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1. A method of expanding in vitro at least one neural stem cell to an expanded population of neural stem cells, wherein each neural stem cell expansion exceeds thirty cell doublings without differentiating, said method comprising:
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dissociating neural stem cells from central nervous system tissue; providing at least one extracellular protein to a culture vessel, wherein the extracellular protein includes at least about 10 μ
g/mL of poly-D-lysine and less than about 1 mg/ml fibronectin;culturing the dissociated neural stem cells in said culture vessel in the absence of serum; adding to the culture vessel at least one growth factor; and passaging the cultured cells prior to confluence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification