METHOD FOR EXTRACELLULAR MATRIX MEDIATED DIFFERENTIATION AND PROLIFERATION OF STEM CELLS

• US 20100285585A1
• Filed: 12/05/2008
• Published: 11/11/2010
• Est. Priority Date: 12/06/2007
• Status: Active Grant

• Current Assignee: Agency For Science Technology and Research
• Original Assignee: Agency For Science Technology and Research
• Inventors: Narayanan, Karthikeyan, Schumacher, Karl M., Ying, Jackie Y., Wan, Andrew Chwee Aun
• Granted Patent: US 8,372,643 B2
• Time in Patent Office: Days
• US Class Current: 435/377
• CPC Class Codes: C12N 2502/13 connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
• CPC Class Codes: C12N 2502/22 pancreatic cells
• CPC Class Codes: C12N 2502/256 Renal cells
• CPC Class Codes: C12N 2533/90 Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
• CPC Class Codes: C12N 5/0606 Pluripotent embryonic cells, e.g. embryonic stem cells [ES] embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696
• CPC Class Codes: C12N 5/0663 Bone marrow mesenchymal stem cells (BM-MSC)

Abstract

There is provided a method of culturing a stem cell on extracellular matrix extracted from support cells and in a stem cell culture medium comprising medium conditioned by the support cells

Claims

1. A method of directing differentiation of a stem cell into a pre-selected type, said method comprising culturing the stem cell on extracellular matrix extracted from differentiated support cells and in a stem cell culture medium comprising medium conditioned by the differentiated support cells to promote differentiation of the stem cell into said pre-selected cell type.

2. The method according to claim 1 wherein said stem cell culture medium is free from the differentiated support cells.

3. The method according to claim 1 wherein the differentiated support cells are proximal tubule cells, pancreatic insulin-secreting cells, osteoblasts, neuronal cells, glial cells, hepatocytes or myoblasts.

4. The method according to claim 3 wherein the differentiated support cells are human proximal tubule cells.

5. The method according to claim 3 wherein the differentiated support cells are pancreatic insulin-secreting cells.

6. The method according to claim 3 wherein the differentiated support cells are osteoblasts.

7. The method according to claim 1 wherein the stem cell is an embryonic stem cell or an mesenchymal stem cell.

8. The method according to claim 7 wherein the stem cell is a human embryonic stem cell.

9. The method according to claim 7 wherein the stem cell is a human mesenchymal stem cell.

10. The method according to claim 1 wherein the stem cell is cultured in a tissue culture plate or culture flask.

11. The method according to claim 1 wherein the stem cell is cultured in a bioartificial device.

12. The method according to claim 11 wherein the bioartificial device is a bioartificial tubule assist device and the differentiated support cells are human proximal tubule cells.

13. The method according to claim 1 wherein the stem cell culture medium comprises about 45% (v/v) to about 55% (v/v) medium conditioned by the differentiated support cells.