DETECTION METHODS
First Claim
1. A method for detecting an analyte A in a sample, comprising:
- (i) incubating an incubation mixture comprising a sample with an analyte A-specific binding partner R1, which is associated with a solid phase, an analyte A-specific binding partner R2, which is associated with a label L1, and an analyte A-specific binding partner R3, which is associated with a label L2, wherein binding partners R2 and R3 are selected such that saturation of analyte A-binding sites of the binding partner R2 requires a) a higher analyte A concentration, b) a longer incubation, or c) a higher analyte A concentration and a longer incubation, than does saturation of analyte A-binding sites of the binding partner R3; and
(ii) determining an L1-dependent measurement signal at time T1 and an L2-dependent measurement signal or an L1 plus L2-dependent measurement signal at time T2, wherein time T1 is earlier than time T2, time T2 is after addition of label L2, and time T1 occurs before 30% of time from addition of label L2 to time T2 has elapsed;
or determining an L1-dependent measurement signal using a first measurement method and an L2-dependent measurement signal or an L1 plus L2-dependent measurement signal using a second measurement method, wherein the first and second measurement methods are different.
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Accused Products
Abstract
The invention relates to a method for quantitatively or qualitatively detecting an analyte in a sample, with the sample being incubated, for the purpose of avoiding, diminishing and/or detecting the high-dose hook effect, with an analyte-specific binding partner R1, which is associated with a solid phase, an analyte-specific binding partner R2, which is associated with a label L1, and an analyte-specific binding partner R3, which is associated with a label L2, and the L1-dependent measurement signal being determined either at a different time from the L2-dependent or L1 plus L2-dependent measurement signal or using a different measurement method.
34 Citations
56 Claims
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1. A method for detecting an analyte A in a sample, comprising:
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(i) incubating an incubation mixture comprising a sample with an analyte A-specific binding partner R1, which is associated with a solid phase, an analyte A-specific binding partner R2, which is associated with a label L1, and an analyte A-specific binding partner R3, which is associated with a label L2, wherein binding partners R2 and R3 are selected such that saturation of analyte A-binding sites of the binding partner R2 requires a) a higher analyte A concentration, b) a longer incubation, or c) a higher analyte A concentration and a longer incubation, than does saturation of analyte A-binding sites of the binding partner R3; and (ii) determining an L1-dependent measurement signal at time T1 and an L2-dependent measurement signal or an L1 plus L2-dependent measurement signal at time T2, wherein time T1 is earlier than time T2, time T2 is after addition of label L2, and time T1 occurs before 30% of time from addition of label L2 to time T2 has elapsed;
or determining an L1-dependent measurement signal using a first measurement method and an L2-dependent measurement signal or an L1 plus L2-dependent measurement signal using a second measurement method, wherein the first and second measurement methods are different. - View Dependent Claims (2, 3, 4, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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5. A method for detecting an analyte A in a sample, comprising:
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(i) incubating an incubation mixture comprising a sample with an analyte A-specific binding partner R1, which is associated with a solid phase, an analyte A-specific binding partner R2, which is associated with a label L1, and an analyte A-specific binding partner R3, which is associated with a first member of a specific binding pair, wherein binding partners R2 and R3 are selected such that saturation of analyte A-binding sites of the binding partner R2 requires a) a higher analyte A concentration, b) a longer incubation, or c) a higher analyte A concentration and a longer incubation, than does saturation of analyte A-binding sites of the binding partner R3; (ii) incubating the incubation mixture of (i) with a label L2, which is associated with a second member of the specific binding pair; and (iii) determining an L1-dependent measurement signal at time T1 and an L2-dependent measurement signal or an L1 plus L2-dependent measurement signal at time T2, wherein time T1 is earlier than time T2, time T2 is after addition of label L2, and time T1 occurs before 30% of time from addition of label L2 to time T2 has elapsed;
or determining an L1-dependent measurement signal using a first measurement method and an L2-dependent measurement signal or an L1 plus L2-dependent measurement signal using a second measurement method, wherein the first and second measurement methods are different. - View Dependent Claims (32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45)
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6. (canceled)
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19. A method for detecting an analyte A in a sample, comprising:
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(i) incubating an incubation mixture comprising a sample with an analyte A-specific binding partner R1, which is associated with a solid phase, an analyte A-specific binding partner R2, which is associated with a label L1, and an analyte A-specific binding partner R3, which is associated with a first member of a specific binding pair, and a label L2, which is associated with a second member of the specific binding pair, wherein binding partners R2 and R3 are selected such that saturation of analyte A-binding sites of the binding partner R2 requires a) a higher analyte A concentration, b) a longer incubation, or c) a higher analyte A concentration and a longer incubation, than does saturation of analyte A-binding sites of the binding partner R3; and (ii) determining an L1-dependent measurement signal at time T1 and an L2-dependent measurement signal or an L1 plus L2-dependent measurement signal at time T2, wherein time T1 is earlier than time T2, time T2 is after addition of label L2, and time T1 occurs before 30% of time from addition of label L2 to time T2 has elapsed;
or determining an L1-dependent measurement signal using a first measurement method and an L2-dependent measurement signal or an L1 plus L2-dependent measurement signal using a second measurement method, wherein the first and second measurement methods are different. - View Dependent Claims (20, 21, 22, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56)
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23. A test kit for detecting an analyte A in a sample, comprising:
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an analyte A-specific binding partner R1, which is associated with a solid phase; an analyte A-specific binding partner R2, which is associated with a label L1; and an analyte A-specific binding partner R3, which is associated with a label L2; wherein saturation, in an incubation mixture of a sandwich test, of analyte A-binding sites of the binding partner R2 takes place at a higher analyte A concentration, at a later time in the incubation, or at a higher analyte A concentration and at a later time in the incubation, than does saturation of analyte A-binding sites of the binding partner R3. - View Dependent Claims (24, 25, 26)
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27. A test kit for detecting an analyte A in a sample, comprising:
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an analyte A-specific binding partner R1, which is associated with a solid phase; an analyte A-specific binding partner R2, which is associated with a label L1; an analyte A-specific binding partner R3, which is associated with a member X of a specific binding pair; and a label L2, which is associated with the binding pair member Y, corresponding to X, of the specific binding pair; wherein saturation, in an incubation mixture of a sandwich test, of analyte A-binding sites of the binding partner R2 takes place at a higher analyte A concentration, at a later time in the incubation, or at a higher analyte A concentration and at a later time in the incubation, than does saturation of analyte A-binding sites of the binding partner R3. - View Dependent Claims (28, 29, 30, 31)
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Specification