Method of collecting placental cells
First Claim
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1. A method of collecting placental cells from a mammalian placenta, comprising perfusing said mammalian placenta with a perfusion solution in an amount and for a time sufficient to collect a detectable amount of said placental cells, wherein said placenta is contained within a sterile bag during said perfusing.
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Abstract
Provided herein are improved methods of collecting and recovering placental cells from a mammalian placenta, comprising, e.g., perfusing a mammalian placenta in a closed system such as a sterile bag and folding the placenta at least once during perfusion. Such folding, and perfusion, can be performed mechanically.
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20 Claims
- 1. A method of collecting placental cells from a mammalian placenta, comprising perfusing said mammalian placenta with a perfusion solution in an amount and for a time sufficient to collect a detectable amount of said placental cells, wherein said placenta is contained within a sterile bag during said perfusing.
- 2. A method of collecting placental cells from a mammalian placenta, comprising perfusing said mammalian placenta with a perfusion solution in an amount and for a time sufficient to collect a detectable amount of said placental cells, wherein a first portion of said placenta is folded at least once towards a second portion of said placenta during said perfusing, and wherein said first portion of said placenta is different from said second portion of said placenta.
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