METHODS OF MAKING ENHANCED, AUTOLOGOUS FAT GRAFTS
First Claim
1. A method of making an enhanced, autologous fat graft comprising supplementing a first portion of extracted adipose tissue with an additive and a disaggregated population of adipose-derived cells that comprise stem cells prepared from a second portion of extracted adipose tissue, so as to make said enhanced, autologous fat graft.
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Accused Products
Abstract
Cells present in processed lipoaspirate tissue are used to treat patients. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of stem cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the stem cells are not exposed to an external environment prior to being administered to a patient. Compositions that are administered to a patient include a mixture of adipose tissue and stem cells so that the composition has a higher concentration of stem cells than when the adipose tissue was removed from the patient.
15 Citations
19 Claims
- 1. A method of making an enhanced, autologous fat graft comprising supplementing a first portion of extracted adipose tissue with an additive and a disaggregated population of adipose-derived cells that comprise stem cells prepared from a second portion of extracted adipose tissue, so as to make said enhanced, autologous fat graft.
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12. A method of making an enhanced, autologous fat graft comprising:
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(a) disaggregating a first portion of extracted adipose tissue with an enzyme or mechanical force to generate a cell suspension; (b) concentrating the cells in said cell suspension by buoyant density sedimentation, filtration or centrifugation to obtain a concentrated population of adipose-derived cells that comprises stem cells; and (c) mixing the concentrated population of adipose-derived cells that comprises stem cells with a second portion of extracted adipose tissue and an additive, so as to make said enhanced, autologous fat graft. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19)
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Specification