OPTICAL READER SYSTEM FOR SUBSTRATES HAVING AN OPTICALLY READABLE CODE

• US 20110114729A1
• Filed: 01/26/2011
• Published: 05/19/2011
• Est. Priority Date: 08/20/2002
• Status: Active Grant

• Current Assignee: Illumina Incorporated
• Original Assignee: Illumina Incorporated
• Inventors: LI, TUO, FOURNIER, DAVID R., KERSEY, ALAN D., PUTNAM, MARTIN A., MOON, JOHN A.
• Granted Patent: US 8,333,325 B2
• Time in Patent Office: Days
• US Class Current: 235/454
• CPC Class Codes: G06K 19/06084 the marking being based on nanoparticles or microbeads
• CPC Class Codes: G06K 19/06178 the marking having a feature size being smaller than can be seen by the unaided human eye
• CPC Class Codes: G06K 2019/06225 using wavelength selection, e.g. colour code
• CPC Class Codes: G06K 7/12 using a selected wavelength, e.g. to sense red marks and ignore blue marks

Abstract

An optical reader system including a source light assembly that has a code-reading beam and a fluorescence-excitation beam that are configured to illuminate encoded substrates. The substrates have optically readable codes that provide output signals when the code-reading beam is incident thereon. The output signals are indicative of the codes. The reader system also includes a fluorescence detector that is configured to detect fluorescent signals from the substrates and code pickup optics that are configured to project the output signals from the optically readable codes onto a Fourier plane. The reader system also includes a code detector that is positioned to detect the output signals in the Fourier plane.

Claims

1. An optical reader system comprising:

a source light assembly including a code-reading beam and a fluorescence-excitation beam that are configured to illuminate encoded substrates, the substrates having optically readable codes that provide output signals when the code-reading beam is incident thereon, the output signals being indicative of the codes;

a fluorescence detector configured to detect fluorescent signals from the substrates;

code pickup optics configured to project the output signals from the optically readable codes onto a Fourier plane; and

a code detector positioned to detect the output signals in the Fourier plane.

2. The system in accordance with claim 1 wherein the fluorescent signals are indicative of analytes being selectively bound to probes on corresponding substrates.

3. The system in accordance with claim 1 further comprising a computing system, the computing system receiving data based on the optical signals and the fluorescent signals, the computing system determining the codes of the substrates and levels of fluorescence from the substrates.

4. The system in accordance with claim 1 wherein the substrates have an elongated body extending along a longitudinal axis, the code-reading beam being incident upon the substrates at a non-orthogonal or perpendicular angle with respect to the longitudinal axis.

5. The system in accordance with claim 1 further comprising a holder configured to align the substrates.

6. The system in accordance with claim 1 further comprising a holder configured to hold the substrates in stationary positions with respect to each other.

7. The system in accordance with claim 1 further comprising the substrates, wherein the codes of the substrates comprise diffraction gratings.

8. The system in accordance with claim 1 wherein the code detector comprises a camera.

9. The system in accordance with claim 1 wherein the fluorescence detector comprises a photomultiplier tube.

10. The system in accordance with claim 1, wherein the code detector detects images of Fourier transforms of resultant refractive index variations in the substrates.

11. A method comprising:

illuminating encoded substrates with a code-reading beam and a fluorescence-excitation beam, the substrates having optically readable codes that provide output signals when the code-reading beam is incident thereon, the output signals being indicative of the codes, and the substrates further having fluorescent molecules that provide fluorescent signals when the fluorescence-excitation beam is incident thereon;

projecting the output signals onto a Fourier plane;

detecting the output signals in the Fourier plane; and

detecting the fluorescent signals.

12. The method in accordance with claim 11 further comprising:

determining the codes of the substrates; and

determining levels of fluorescence of the substrates.

13. The method in accordance with claim 11 further comprising aligning the substrates before said illuminating.

14. The method in accordance with claim 11 wherein the substrates have an elongated body extending along a longitudinal axis, the code-reading beam being incident upon the substrates at a non-orthogonal or perpendicular angle with respect to the longitudinal axis.

15. The method in accordance with claim 11 wherein the code detector comprises a camera.

16. The method in accordance with claim 11 wherein the fluorescence detector comprises a photomultiplier tube.

17. The method in accordance with claim 11 wherein said illuminating includes illuminating the substrates with the code-reading beam and the fluorescent-excitation beam at different times.

18. The method in accordance with claim 11, wherein said detecting the optical signals includes detecting images of Fourier transforms of resultant refractive index variations in the substrates.

19. The method in accordance with claim 11 wherein the codes of the substrates comprise diffraction gratings.

20. The method in accordance with claim 11 wherein the code-reading and fluorescence-excitation beams illuminate the substrates at different times.