Biosensor

• US 20110180404A1
• Filed: 12/21/2010
• Published: 07/28/2011
• Est. Priority Date: 03/29/2000
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Healthcare Company Limited (PHC Holdings Corporation)
• Inventors: Nakaminami, Takahiro, Nakayama, Junko, Tokunaga, Hiroyuki, Yamanishi, Eriko, Fujiwara, Masaki, Miyazaki, Shoji
• Granted Patent: US 8,673,127 B2
• Time in Patent Office: Days
• US Class Current: 204/403.14
• CPC Class Codes: C12Q 1/003 Functionalisation

Abstract

In a biosensor for measuring a specific substance in a liquid sample, one or a combination of sugar alcohol, metallic salt, organic acid or organic acid salt which has at least one carboxyl group in a molecule, and organic acid or organic acid salt which has at least one carboxyl group and one amino group in a molecule, is included in a reagent layer provided on electrodes, thereby providing a highly-accurate biosensor which is excellent in stability and has high response (sensitivity, linearity) of the sensor to the substrate concentration.

Claims

1-24. -24. (canceled)

25. A biosensor for measuring the concentration of a specific substance in a sample solution, the biosensor comprising

electrodes comprising at least a working electrode and a counter electrode provided on an insulating support;

and

a reagent layer which reacts with the specific substance in the sample solution;

wherein the reagent layer comprises an enzyme, an electron transfer agent, and an organic acid or organic acid salt which has at least one carboxyl group, and

the organic acid is aliphatic carboxylic acid, carbocyclic carboxylic acid, heterocyclic carboxylic acid, or a substitution product or derivative of the organic acid.

26. The biosensor as defined in claim 25, wherein

the carboxylic acid is one of malonic acid, succinic acid, glutaric acid, adipic acid, maleic acid, fumaric acid, benzoic acid, phthalic acid, isophthalic acid, terephthalic acid, 2-phthalic acid, nicotinic acid, isonicotinic acid, and their salt, or a combination thereof.

27. The biosensor as defined in claim 25, wherein

the enzyme included in the reagent layer is glucose oxidase or glucose dehydrogenase.

28. The biosensor as defined in claim 26, wherein

the enzyme included in the reagent layer is glucose oxidase or glucose dehydrogenase.

29. The biosensor as defined in claim 25, wherein

the reagent layer comprises a hydrophilic polymer.

30. The biosensor as defined in claim 26, wherein

the reagent layer comprises a hydrophilic polymer.

31. The biosensor as defined in claim 27, wherein

the reagent layer comprises a hydrophilic polymer.

32. The biosensor as defined in claim 28, wherein

the reagent layer comprises a hydrophilic polymer.

33. A biosensor for measuring the concentration of a specific substance in a sample solution, the biosensor comprising

electrodes comprising at least a working electrode and a counter electrode provided on an insulating support;

and

a reagent layer which reacts with the specific substance in the sample solution;

wherein the reagent layer includes an enzyme, an electron transfer agent, and an organic acid or organic acid salt which has at least one carboxyl group and one amino group, and

the organic acid is an amino acid, or a substitution product or derivative of an amino acid.

34. The biosensor as defined in claim 33, wherein

the amino acid or the organic acid is one of alanine, valine, leucine, isoleucine, serine, threonine, methionine, asparagine, glutamine, arginine, lysine, histidine, phenylalanine, tryptophan, proline, or their salt, or sarcosine and betaine, or a combination thereof.

35. The biosensor as defined in claim 33, wherein

the enzyme included in the reagent layer is glucose oxidase or glucose dehydrogenase.

36. The biosensor as defined in claim 34, wherein

the enzyme included in the reagent layer is glucose oxidase or glucose dehydrogenase.

37. The biosensor as defined in claim 33, wherein

the reagent layer comprises a hydrophilic polymer.

38. The biosensor as defined in claim 34, wherein

the reagent layer comprises a hydrophilic polymer.

39. The biosensor as defined in claim 35, wherein

the reagent layer comprises a hydrophilic polymer.

40. The biosensor as defined in claim 36, wherein

the reagent layer comprises a hydrophilic polymer.