Continuous Testing Method

• US 20110203354A1
• Filed: 05/02/2011
• Published: 08/25/2011
• Est. Priority Date: 04/30/2008
• Status: Abandoned Application

• Current Assignee: Crystalvue Medical Corporation
• Original Assignee: Crystalvue Medical Corporation
• Inventors: Yang, Yuh-Shyong, Liu, Cheng-Hsien, Hsu, Long, Wang, William, Chou, Chung-Cheng
• Application Number:
  US13/098,599
• Publication Number:
  US 20110203354A1
• Time in Patent Office: Days
• US Class Current: 73/61.72
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2400/0424 Dielectrophoretic forces
• CPC Class Codes: B01L 2400/0427 Electrowetting
• CPC Class Codes: B01L 2400/0454 radiation pressure, optical tweezers
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 3/502792 for moving individual droplets on a plate, e.g. by locally altering surface tension
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: B03C 2201/26 for use in medical applications
• CPC Class Codes: B03C 2201/32 Checking the quality of the result or the well-functioning of the device
• CPC Class Codes: B03C 5/005 Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength B03C5/02 takes precedence; electrophoresis B01D57/02
• CPC Class Codes: B03C 5/026 using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
• CPC Class Codes: G01N 2021/0325 Cells for testing reactions, e.g. containing reagents
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/058 Flat flow cell
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/31 Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry G01N21/72 takes precedence
• CPC Class Codes: G01N 2201/062 LED's
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence

Abstract

A continuous testing method for testing the concentration of a target object in a fluid is provided. The method comprises the following steps. A focused light is provided in the fluid to separate the target object from a non-target object in the fluid by changing the movement direction of the target object and the non-target object. The fluid having separated out the non-target object is enabled to react with a reagent. A signal is provided to pass through the fluid having reacted with the reagent. The signal passing through the fluid is received and an electronic signal is outputted corresponding to the input signal. The concentration of the target object is acquired according to the electronic signal.

Claims

1. A continuous testing method for testing the concentration of a target object in a fluid, comprising:

providing a focused light in the fluid to separate the target object from a non-target object in the fluid by changing the movement direction of the target object and the non-target object;

enabling the fluid having separated out the non-target object to react with a reagent;

providing a signal to pass through the fluid having reacted with the reagent;

receiving the signal passing through the fluid and outputting an electronic signal corresponding to the input signal; and

acquiring the concentration of the target object according to the electronic signal.

2. The continuous testing method according to claim 1, wherein in the step of separating the target object and the non-target object, a dielectrophoretic force (DEP force) is generated in the fluid to separate the target object from the non-target object in the fluid.

3. The continuous testing method according to claim 1, wherein in the step of enabling the fluid having separated out the non-target object to react with the reagent, the fluid and the reagent are received in at least one reaction channel by an electrowetting effect.

4. The continuous testing method according to claim 3, wherein in the step of enabling the fluid having separated out the non-target object to react with the reagent, the fluid and the reagent in the at least one reaction channel further form a focusing liquid drop by the electrowetting effect.

5. The continuous testing device according to claim 3, wherein in the step of enabling the fluid having separated out the non-target object to react with the reagent, a wave signal is provided to the at least one reaction channel to generate the electrowetting effect.