METHOD FOR FORMING A MOLECULARLY IMPRINTED POLYMER BIOSENSOR

• US 20110241260A1
• Filed: 09/24/2010
• Published: 10/06/2011
• Est. Priority Date: 09/16/2009
• Status: Active Grant

• Current Assignee: National Tsing Hua University
• Original Assignee: National Tsing Hua University
• Inventors: CHANG, Po-Hsiang, HONG, Chien-Chong, LIN, Chih-Chung
• Granted Patent: US 8,734,890 B2
• Time in Patent Office: Days
• US Class Current: 264/425
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 40/00 Manufacture or treatment of nanostructures
• CPC Class Codes: G01N 2600/00 Assays involving molecular imprinted polymers/polymers created around a molecular template
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G03F 7/0002 Lithographic processes using patterning methods other than those involving the exposure to radiation, e.g. by stamping
• CPC Class Codes: G03F 7/027 Non-macromolecular photopolymerisable compounds having carbon-to-carbon double bonds, e.g. ethylenic compounds G03F7/075 takes precedence
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

A method for forming a molecularly imprinted polymer biosensor includes: (a) preparing a reaction solution including an imprinting molecule, a functional monomer, an initiator, and a crosslinking agent; (b) disposing the reaction solution in a space between upper and lower substrates each of which is made of a light-transmissible material; (c) disposing on the upper substrate a photomask having a patterned hole; (d) irradiating the reaction solution through the patterned hole of the photomask and the upper substrate so that the reaction solution undergoes polymerization to form a polymer between the upper and lower substrates; (e) removing the upper substrate after the polymer is formed on the lower substrate; and (f) extracting the imprinting molecule from the polymer so that a patterned molecularly imprinted polymer film is formed on the lower substrate.

Claims

1. A method for forming a molecularly imprinted polymer biosensor, comprising:

(a) preparing a reaction solution including an imprinting molecule, a functional monomer, an initiator, and a crosslinking agent;

(b) disposing the reaction solution in a space between upper and lower substrates each of which is made of a light-transmissible material;

(c) disposing on the upper substrate a photomask having a patterned hole;

(d) irradiating the reaction solution through the patterned hole of the photomask and the upper substrate so that the reaction solution undergoes polymerization to form a polymer between the upper and lower substrates;

(e) removing the upper substrate after the polymer is formed on the lower substrate; and

(f) extracting the imprinting molecule from the polymer so that a patterned molecularly imprinted polymer film is formed on the lower substrate.

2. The method of claim 1, further comprising bonding a microchannel substrate to the lower substrate having the patterned molecularly imprinted polymer film.

3. The method of claim 1, wherein the imprinting molecule is anesthetic.

4. The method of claim 3, wherein the anesthetic is propofol.

5. The method of claim 1, wherein the step (d) is conducted with an irradiation energy ranging from 16 J/cm² to 72 J/cm².

6. The method of claim 1, wherein the light-transmissible material is a cyclic olefin copolymer.

7. The method of claim 1, wherein the molecularly imprinted polymer film has a thickness ranging from 25 μ

m to 75 μ

m.

8. The method of claim 1, wherein the molecularly imprinted polymer film has a plurality of pores with a pore size ranging from 10 nm to 30 nm.