Electrochemical Biosensor Structure and Measuring Method Using the Same

• US 20110272295A1
• Filed: 05/18/2009
• Published: 11/10/2011
• Est. Priority Date: 10/16/2008
• Status: Active Grant

• Current Assignee: Andong National University Industry Academic Cooperation Foundation
• Original Assignee: Andong National University Industry Academic Cooperation Foundation
• Inventors: Kim, Tae Wan, Suh, Ho Chul, Kim, Kyu Jun, Lee, Young Tae
• Granted Patent: US 8,888,972 B2
• Time in Patent Office: Days
• US Class Current: 205/792
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

The present invention provides there is provided an electrochemical biosensor electrode structure that includes: a working electrode and a reference electrode used as electrodes for sample measurement being arranged separately from each other in lengthwise direction of a sample insertion path, the working electrode and the reference electrode each having at least one projection and at least one recess alternately arranged on a portion thereof corresponding to the sample insertion path, the projection of the working electrode being correspondingly adjacent to the recess of the reference electrode, the recess of the working electrode being correspondingly adjacent to the projection of the reference electrode; and at least two sample recognition electrodes used as electrodes for sample recognition being separated from each other and arranged adjacent and parallel to the working electrode and the reference electrode. The present invention minimizes the effect of the double layer capacitance and independently applies the sample recognition signal to accurately detect the sample insert time and velocity.

Claims

1. An electrochemical biosensor electrode structure comprising:

a working electrode and a reference electrode used as electrodes for sample measurement being arranged separately from each other in lengthwise direction of a sample insertion path,

the working electrode and the reference electrode each having at least one projection and at least one recess alternately arranged on a portion thereof corresponding to the sample insertion path, the projection of the working electrode being correspondingly adjacent to the recess of the reference electrode, the recess of the working electrode being correspondingly adjacent to the projection of the reference electrode; and

at least two sample recognition electrodes used as electrodes for sample recognition being separated from each other and arranged adjacent and parallel to the working electrode and the reference electrode.

2. The electrochemical biosensor electrode structure as claimed in claim 1, wherein the at least two sample recognition electrodes are arranged between the working electrode and the reference electrode, the ends of the at least two sample recognition electrodes being adjacent to the end of the sample insertion path.

3. The electrochemical biosensor electrode structure as claimed in claim 2, wherein the at least two sample recognition electrodes recognize a sample in response to a sample recognition signal, the sample recognition signal being applied independent of a sample measurement signal applied to the reference electrode or the working electrode to recognize the sample.

4. The electrochemical biosensor electrode structure as claimed in claim 1, further comprising:

a strip recognition electrode for sensing the moment that a biosensor having the biosensor electrode is inserted into a measurement device to take a measurement.

5. The electrochemical biosensor electrode structure as claimed in claim 1, wherein the working electrode and the reference electrode are separated from each other at a separation distance of 50 to 120 μ

m in a portion thereof corresponding to the sample insertion path.

6. A structure for electrochemical biosensor comprising:

a bottom plate comprising electrodes for sample measurement, and at least two sample recognition electrodes arranged separately from the electrodes for sample measurement on an insulating substrate, the electrodes for sample measurement including a working electrode and a reference electrode,

the working electrode and the reference electrode being arranged separately from each other in lengthwise direction of a sample insertion path,

the working electrode and the reference electrode each having at least one projection and at least one recess alternately arranged on a portion thereof corresponding to the sample insertion path, the projection of the working electrode being correspondingly adjacent to the recess of the reference electrode, the recess of the working electrode being correspondingly adjacent to the projection of the reference electrode,

the at least two sample recognition electrodes being separated from each other and arranged adjacent and parallel to the working electrode and the reference electrode;

a middle plate having a double-coated adhesive insulating film structure with the sample insertion path formed thereon; and

a top plate having at least two air exhaust holes being formed on the insulating substrate in correspondence to the end of the sample insertion path, p1 the at least two air exhaust holes being provided correspondingly to the ends of the at least two sample recognition electrodes to vertically overlap the ends of the at least two sample recognition electrodes.

7. The structure for electrochemical biosensor as claimed in claim 6, wherein the at least two sample recognition electrodes recognize a sample in response to a sample recognition signal, the sample recognition signal being applied independent of a sample measurement signal applied to the reference electrode or the working electrode to recognize of the sample.

8. The structure for electrochemical biosensor as claimed in claim 6, wherein a first sample recognition electrode as the one of the at least two sample recognition electrodes is provided for applying a sample recognition signal,

a second sample recognition electrode as the other one of the at least two sample recognition electrodes being provided for receiving a sample arrival signal.

9. The structure for electrochemical biosensor as claimed in claim 6, wherein the working electrode and the reference electrode are separated from each other at a separation distance of 50 to 120 μ

m in a portion thereof corresponding to the sample insertion path.

10. A sample measurement method using a biosensor, comprising:

(a) preparing the biosensor having electrodes for sample measurement, and at least two sample recognition electrodes provided separately from the electrodes for sample measurement, the electrodes for sample measurement including a working electrode and a reference electrode,

the at least two sample recognition electrodes receiving a sample recognition signal controlled separately from a sample measurement signal applied to the working electrode and the reference electrode;

(b) applying the sample recognition signal to the one of the at least two sample recognition electrodes to determine whether a sample has arrived or not, the sample recognition signal being generated and controlled independent of the sample measurement signal; and

(c) if the sample has arrived, applying the sample measurement signal to the working electrode and the reference electrode and measuring a response signal to the sample.

11. The sample measurement method using a biosensor as claimed in claim 10, wherein in the step (b), whether the sample has arrived is determined by receiving a sample arrival signal through the other one of the at least two sample recognition electrodes.

12. The sample measurement method using a biosensor as claimed in claim 11, wherein the working electrode and the reference electrode are arranged separately from each other in lengthwise direction of a sample insertion path, the working electrode and the reference electrode each having at least one projection and at least one recess alternately arranged on a portion thereof corresponding to the sample insertion path, the projection of the working electrode being correspondingly adjacent to the recess of the reference electrode, the recess of the working electrode being correspondingly adjacent to the projection of the reference electrode.

13. A method for fabricating an electrochemical biosensor electrode, comprising:

forming a first conductive thin film adhesively on an insulating substrate by lamination;

coating a photosensitivity dry film on the first conductive thin film by lamination, and performing a photo and etching process to form an electrode pattern comprising a material of the first conductive thin film, the electrode pattern integrally having at least two sample recognition electrodes and electrodes for sample measurement, the electrodes for sample measurement including a working electrode and a reference electrode,

the working electrode and the reference electrode being arranged separately from each other in lengthwise direction of a sample insertion path,

the working electrode and the reference electrode each having at least one projection and at least one recess alternately arranged on a portion thereof corresponding to the sample insertion path, the projection of the working electrode being correspondingly adjacent to the recess of the reference electrode, the recess of the working electrode being correspondingly adjacent to the projection of the reference electrode; and

forming a second conductive thin film on the surface of the electrode pattern, the second conductive thin film having a higher conductivity than the electrode pattern.

14. The method as claimed in claim 13, wherein the insulating substrate is a polymer film comprising at least one material selected from polyester, polycarbonate, polystylene, polyimide, polyvinylchloride, polyethylene, or polyethyleneterephthalate (PET).

15. The method as claimed in claim 13, wherein the first conductive thin film comprises a material of copper or copper alloy.

16. The method as claimed in claim 13, further comprising:

forming a nickel or chrome layer between the first and second conductive thin films by plating to enhance adhesion strength between the first and second conductive thin films.

17. The method as claimed in claim 13, wherein the second conductive thin film is formed through gold or platinum plating.

18. The method as claimed in claim 13, wherein the at least two sample recognition electrodes include two sample recognition electrodes adjacent to the working electrode or the reference electrode.

19. The method as claimed in claim 13, wherein the working electrode and the reference electrode are separated from each other at a separation distance of 50 to 120 μ

m in a portion thereof corresponding to the sample insertion path.

20. The method as claimed in claim 13, wherein the insulating substrate with the working electrode, the reference electrode and the at least two sample recognition electrodes formed thereon is a bottom plate of the biosensor comprising top, middle and bottom plates,

the method further comprising:

after preparation of the bottom plate of the biosensor, forming the middle plate of a double-coated adhesive insulating film structure with the sample insertion path formed thereon; and

forming the top plate having at least two air exhaust holes corresponding to the end of the sample insertion path in the insulating substrate, the at least two air exhaust holes being provided correspondingly to the ends of the at least two sample recognition electrodes to vertically overlap the ends of the at least two sample recognition electrodes.