HETEROGENEOUS INSEMINATE SYSTEM

• US 20110275052A1
• Filed: 07/18/2011
• Published: 11/10/2011
• Est. Priority Date: 08/01/2002
• Status: Active Grant

• Current Assignee: XY, LLC (Inguran LLC)
• Original Assignee: XY, LLC (Inguran LLC)
• Inventors: Rosenstein, Maurice A., Moreno, Juan F., Schenk, John L., Seidel, George E., Suh, Tae Kwang
• Granted Patent: US 8,486,618 B2
• Time in Patent Office: Days
• US Class Current: 435/2
• CPC Class Codes: A01K 2227/10 Mammal
• CPC Class Codes: A61D 19/00 Instruments or methods for reproduction or fertilisation
• CPC Class Codes: C12N 2517/10 Conditioning of cells for in vitro fecondation or nuclear transfer

Abstract

A heterogeneous inseminate including a first amount of sperm of a first animal and a second amount of sperm of a second animal of the same species, the first amount of sperm and the second amount of sperm sex-selected sperm of the same sex, useful in the in-vivo or in-vitro fertilization of an egg of a female animal of the same species for the production of sex-selected embryos and sex-selected offspring.

Claims

1-22. -22. (canceled)

23. A method of producing a heterogeneous inseminate, comprising the steps of:

a) obtaining a first amount of sperm of a first animal;

b) obtaining a second amount of sperm of a second animal, said first animal and said second animal of the same species, wherein said first amount of sperm of said first animal or said second amount of sperm of said second animal comprises sex-selected sperm; and

c) combining said first amount of sperm of said first animal with said second amount of sperm of said second animal.

24. The method of producing a heterogeneous inseminate of claim 23, wherein said first amount of sperm of said first animal has a greater fertility than said second amount of sperm.

25. The method of producing a heterogeneous inseminate of claim 23, wherein concentration of said first amount of sperm of said first animal in said heterogeneous inseminate has a greater concentration than said second amount of sperm of said second animal.

26. The method of producing a heterogeneous inseminate of claim 23, wherein said first amount of sperm of said first animal comprises an amount of infertile sperm.

27. The method of producing a heterogeneous inseminate of claim 23, wherein said first amount of sperm and said second amount of sperm each comprise said sex-selected sperm.

28. The method of producing a heterogeneous inseminate of claim 27, further comprising the step of selecting sex of said first amount of sperm of said first animal and selecting sex of said second amount of sperm of said second animal prior to said step of combining said first amount of sperm of said first animal with said second amount of sperm of said second animal.

29. The method of producing a heterogeneous inseminate of claim 27, further comprising the step of selecting sex of said first amount of sperm of said first animal and said second amount of sperm of said second animal after said step of combining said first amount of sperm of said first animal with said second amount of sperm of said second animal.

30. The method of producing a heterogeneous inseminate of claim 27, wherein said first amount of sperm of said first animal and/or said second amount of sperm of said second animal comprises frozen-thawed sperm.

31. The method of producing a heterogeneous inseminate of claim 23, further comprising the step of adjusting said first amount of sperm of said first animal in said heterogeneous inseminate to provide a concentration sufficient to increase motility of said second amount of sperm in said heterogeneous inseminate.

32. The method of producing a heterogeneous inseminate of claim 23, further comprising the step of adjusting said first amount of sperm of said first animal in said heterogeneous inseminate to provide a concentration sufficient to increase fertility of said second amount of sperm of said second animal in said heterogeneous inseminate.

33. The method of producing a heterogeneous inseminate of claim 23, wherein said steps of:

a) obtaining a first amount of sperm of a first animal; and

b) obtaining a second amount of sperm of a second animal, said first animal and said second animal of the same species comprises the step of obtaining a plurality of amounts of sperm of a corresponding plurality of animals, said plurality of animals each of the same species of animal.

34. The method of producing a heterogeneous inseminate of claim 33, wherein said plurality of animals is selected from the group consisting of:

three animals, four animals, five animals, and six animals.

35. The method of producing a heterogeneous inseminate of claim 34, wherein one or more of said plurality of amounts of sperm comprises said sex-selected sperm.

36. The method of producing a heterogeneous inseminate of claim 27, wherein said species of animal is selected from the group consisting of:

a mammal, an ayes, and a fish.

37. The method of producing a heterogeneous inseminate of claim 36, wherein said species of animal comprises a bovine mammal and further comprising the step of establishing said second amount of sperm of said second animal in said heterogeneous inseminate at concentration of between about 2×

10⁴ sperm per milliliter and about 4×

10⁶ sperm per milliliter, said second amount of sperm comprising said amount of sex-selected sperm.

38. The method of producing a heterogeneous inseminate of claim 37, further comprising the step of establishing said first amount of sperm of said first animal in said heterogeneous inseminate at a concentration of between about 2×

10⁷ sperm per milliliter and about 8×

10⁷ sperm per milliliter.

39. The method of producing a heterogeneous inseminate of claim 38, wherein said mammal comprises a bovine mammal and further comprising the step of producing said heterogeneous inseminate in a dosage form having a volume of between about one-tenth milliliter and about one milliliter contained in an artificial insemination straw.

40. The method of producing a heterogeneous inseminate of claim 39, further comprising the step of freezing said dosage form having a volume of between about one-tenth milliliter and about one milliliter contained in said artificial insemination straw.

42. The method of producing a heterogeneous inseminate of claim 31, further comprising the step of admixing an amount of cell free seminal fluid prepared from an amount of semen having a greater initial level of fertility than said level of fertility of said first amount of sperm and/or said second amount of sperm.

43. The method of producing a heterogeneous inseminate of claim 23, further comprising the step of ranking fertility of males or ranking fertility of sperm treatments.