EXPRESSION OF HEXOSE KINASE IN RECOMBINANT HOST CELLS
First Claim
1. A recombinant yeast host cell comprising:
- (a) a modification in an endogenous polynucleotide encoding a polypeptide having dual-role hexokinase activity in the host cell wherein the activity of the polypeptide of (a) is reduced or substantially eliminated; and
(b) a heterologous polynucleotide encoding a polypeptide having hexose kinase activity.
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Abstract
The invention relates to a recombinant host cell having (a) a modification in an endogenous polynucleotide encoding a polypeptide having dual-role hexokinase activity; (b) a heterologous polynucleotide encoding a polypeptide having hexose kinase activity; and optionally (c) a modification in an endogenous polynucleotide encoding a polypeptide having pyruvate decarboxylase activity. Additionally, the invention relates to methods of making and using such recombinant host cells including, for example, methods of increasing glucose consumption, methods of improving redox balance, and/or methods of increasing the production of a product of a pyruvate-utilizing pathway.
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Citations
31 Claims
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1. A recombinant yeast host cell comprising:
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(a) a modification in an endogenous polynucleotide encoding a polypeptide having dual-role hexokinase activity in the host cell wherein the activity of the polypeptide of (a) is reduced or substantially eliminated; and (b) a heterologous polynucleotide encoding a polypeptide having hexose kinase activity. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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31. A method for improving the redox balance of a recombinant host cell comprising;
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(i) providing a recombinant host cell comprising (a) a modification in an endogenous polynucleotide encoding a polypeptide having dual-role hexokinase activity; and
(b) a heterologous polynucleotide encoding a polypeptide having hexose kinase activity; and(ii) growing the recombinant host cell of (i) under conditions wherein the heterologous polynucleotide of (b) is expressed in functional form wherein the redox balance of such a recombinant host cell is improved compared to the redox balance of a recombinant host cell comprising (i)(a) but not (i)(b).
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Specification