Methods for dewatering wet algal cell cultures
First Claim
1. A method of dewatering a wet algal cell culture, comprising:
- a. removing at least a portion of liquid in an algal cell culture using a sintered metal tube filter to obtain a wet algal biomass fraction having a lower liquid content than the algal cell culture;
b. recycling at least a portion of the liquid removed from the algal cell culture for use in a different algal cell culture;
c. adding a water miscible solvent set to the wet algal biomass fraction and waiting an amount of time to permit algal cells of the algal biomass fraction to gather; and
d. isolating at least a portion of the gathered algal cells from at least a portion of the solvent set and liquid of the wet algal biomass fraction so that a dewatered algal biomass is generated.
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Abstract
A method of dewatering algae and recycling water therefrom is presented. A method of dewatering a wet algal cell culture includes removing liquid from an algal cell culture to obtain a wet algal biomass having a lower liquid content than the algal cell culture. At least a portion of the liquid removed from the algal cell culture is recycled for use in a different algal cell culture. The method includes adding a water miscible solvent set to the wet algal biomass and waiting an amount of time to permit algal cells of the algal biomass to gather and isolating at least a portion of the gathered algal cells from at least a portion of the solvent set and liquid of the wet algal biomass so that a dewatered algal biomass is generated. The dewatered algal biomass can be used to generated algal products such as biofuels and nutraceuticals.
35 Citations
15 Claims
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1. A method of dewatering a wet algal cell culture, comprising:
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a. removing at least a portion of liquid in an algal cell culture using a sintered metal tube filter to obtain a wet algal biomass fraction having a lower liquid content than the algal cell culture; b. recycling at least a portion of the liquid removed from the algal cell culture for use in a different algal cell culture; c. adding a water miscible solvent set to the wet algal biomass fraction and waiting an amount of time to permit algal cells of the algal biomass fraction to gather; and d. isolating at least a portion of the gathered algal cells from at least a portion of the solvent set and liquid of the wet algal biomass fraction so that a dewatered algal biomass is generated. - View Dependent Claims (2, 3, 4, 5)
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6. A method of dewatering a wet algal cell culture, comprising:
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a. removing at least a portion of liquid in an algal cell culture using at least one of a membrane, centrifugation, a sintered metal tube, dissolved gas flotation, and flocculation to obtain a wet algal biomass fraction having a lower liquid content than the algal cell culture; b. recycling at least a portion of the liquid removed from the algal cell culture for use in a different algal cell culture; c. adding a water miscible solvent set to the wet algal biomass fraction and waiting an amount of time to permit algal cells of the algal biomass fraction to gather; and d. isolating at least a portion of the gathered algal cells from at least a portion of the solvent set and liquid of the wet algal biomass fraction so that a dewatered algal biomass is generated. - View Dependent Claims (7, 8, 9, 10)
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11. A method of dewatering a wet algal cell culture, comprising:
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a. removing at least a portion of liquid in a wet algal cell culture to obtain a wet algal biomass fraction having a lower liquid content than the algal cell culture; b. adding a first water miscible solvent set, comprising one or more solvents, to the wet algal biomass fraction; c. generating a substantially liquid phase and a substantially solid phase from the mixture of the wet algal biomass fraction and water miscible solvent set; d. isolating at least a portion of the substantially solid phase; e. adding a second water miscible solvent set, comprising one or more solvents, to the isolated portion of the substantially solid phase; f. isolating at least a portion of algae solids of the mixture of the substantially solid phase and second water miscible solvent set by sedimentation or flotation of the algae solids; and g. recycling at least a portion of at least one of the first and second water miscible solvent set to a subsequent algal cell culture dewatering process. - View Dependent Claims (12, 13, 14, 15)
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Specification