TUBE FOR MEASURING BIO-RELATED SUBSTANCE AND QUANTIFYING SYSTEM

• US 20120094307A1
• Filed: 04/20/2010
• Published: 04/19/2012
• Est. Priority Date: 04/20/2009
• Status: Active Grant

• Current Assignee: Universal Bio Research Co., Ltd (Precision System Science Co., Ltd)
• Original Assignee: Universal Bio Research Co., Ltd (Precision System Science Co., Ltd)
• Inventors: Tajima, Hideji
• Granted Patent: US 9,778,253 B2
• Time in Patent Office: Days
• US Class Current: 435/7.4
• CPC Class Codes: G01N 2021/0325 Cells for testing reactions, e.g. containing reagents
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 33/54313 the carrier being characterised by its particulate form

Abstract

A bio-related substance assay tube 2 comprises a target substance capture bead 3 serving as a first microparticle, compensation bead 4 serving as a second microparticle on which a given amount of a bio-related substance has been immobilized, and a negative control bead 5 serving as a third microparticle for use as a negative control. A mount unit 12 comprises a nozzle communicating with a pump, and the bio-related substance assay tube 2 is mounted in the mount unit 12 to ensure communication with this nozzle. An analyte is introduced into the bio-related substance assay tube 2, followed by labeling the bio-related substance bound to each microparticle to cause light emission. Based on light emission from each microparticle, a calibration curve is prepared or the emission intensity of the first microparticle is compensated to quantify the bio-related substance.

Claims

1. A bio-related substance assay tube, which comprises:

a first microparticle on which a substance capable of binding to a target bio-related substance to be measured is immobilized;

a second microparticle on which the bio-related substance is immobilized in a given amount; and

a third microparticle for use as a negative control;

wherein these microparticles are aligned in the tube.

2. The tube according to claim 1, wherein the second microparticle is a microparticle used for compensation of data measured for the bio-related substance.

3. The tube according to claim 1, wherein the second microparticle comprises a plurality of microparticles on which different amounts of the bio-related substance are immobilized.

4. The tube according to claim 3, wherein the plurality of microparticles are microparticles used for preparation of a calibration curve.

5. The tube according to any one of claims 1 to 3, which further comprises light-shielding members interposed to separate the first to third microparticles.

6. A quantification system for a bio-related substance, which comprises:

the tube according to claim 1;

a detection unit for detecting signals emitted from the microparticles in the tube;

a compensation unit for compensating data of the detected signals by referring to a calibration curve which has been prepared; and

an arithmetic unit for quantifying the bio-related substance based on the compensated data.

7. A quantification system for a bio-related substance, which comprises:

the tube according to claim 1;

a detection unit for detecting signals emitted from the microparticles in the tube;

a calibration curve preparation unit for preparing a calibration curve based on the detected signals; and

an arithmetic unit for quantifying the bio-related substance by referring to the prepared calibration curve.

8. A method for measuring a bio-related substance, which comprises contacting an analyte with the tube according to claim 1, and measuring the target bio-related substance in the analyte.

9. The method according to claim 8, wherein qualitative detection and quantification are simultaneously performed on the target bio-related substance during the measurement.