XYLOSE UTILIZING ZYMOMONAS MOBILIS WITH IMPROVED ETHANOL PRODUCTION IN BIOMASS HYDROLYSATE MEDIUM
First Claim
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1. A recombinant, xylose-utilizing, ethanol-producing microorganism of the genus Zymomonas, having at least one genetic modification in the zmo1432 open reading frame.
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Abstract
Xylose-utilizing, ethanol producing strains of Zymomonas mobilis with improved performance in medium comprising biomass hydrolysate were isolated using an adaptation process. Independently isolated strains were found to have independent mutations in the same coding region. Mutation in this coding may be engineered to confer the improved phenotype.
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Citations
8 Claims
- 1. A recombinant, xylose-utilizing, ethanol-producing microorganism of the genus Zymomonas, having at least one genetic modification in the zmo1432 open reading frame.
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5. A polynucleotide encoding a protein having the amino acid sequence selected from the group consisting of SEQ ID NO:
- 3 and SEQ ID NO;
4.
- 3 and SEQ ID NO;
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6. A polypeptide having the amino acid sequence selected from the group consisting of in SEQ ID NO:
- 3 and SEQ ID NO;
4.
- 3 and SEQ ID NO;
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7. A method for the production of a recombinant Zymonomas ethanologen comprising:
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a) providing a xylose-utilizing, ethanol-producing microorganism of the genus Zymomonas comprising a zmo1432 open reading frame encoding a polypeptide having the amino acid sequence as set forth in SEQ ID NO;
2; andb) introducing a mutation in the zmo1432 open reading frame of a) such that expression of the mutated open reading frame expresses a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO;
3 and SEQ ID NO;
4.
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Specification