DIFFERENTIATING ANALYTES DETECTED USING FAST SCAN CYCLIC VOLTAMMETRY

• US 20130023745A1
• Filed: 07/23/2012
• Published: 01/24/2013
• Est. Priority Date: 07/21/2011
• Status: Active Grant

• Current Assignee: Mayo Foundation For Medical Education And Research (Mayo Clinic)
• Original Assignee: Mayo Foundation For Medical Education And Research (Mayo Clinic)
• Inventors: Lee, Kendall H., Jang, Dong-Pyo, Kim, Inyong
• Granted Patent: US 9,841,403 B2
• Time in Patent Office: Days
• US Class Current: 600/345
• CPC Class Codes: A61B 5/1473 invasive, e.g. introduced into the body by a catheter A61B5/1482 takes precedence
• CPC Class Codes: A61B 5/6868 Brain
• CPC Class Codes: G01N 27/48 using polarography, i.e. measuring changes in current under a slowly-varying voltage

Abstract

This document provides methods and materials involved in differentiating analytes detected using a FSCV method. For example, methods and materials for using paired pulse voltammetry to discriminate analytes based on their adsorption characteristics to an electrode (e.g., a carbon fiber electrode) are provided.

Claims

1. A method for assessing a concentration of an analyte present within an environment, wherein said method comprises:

(a) obtaining voltammetric data for a primary pulse and a secondary pulse of a binary waveform,

(b) determining a primary voltammogram and a secondary voltammogram from said data,

(c) determining a difference between one of said primary voltammogram and said secondary voltammogram, wherein said difference provides an indication about said concentration, and

(d) providing information about said difference or said concentration for display to a user.

2. The method of claim 1, wherein said analyte is a chemical capable of being measured by electrochemistry.

3. The method of claim 1, wherein said environment is brain tissue in vivo.

4. The method of claim 1, wherein said environment is human brain tissue in vivo.

5. The method of claim 1, wherein the repetition time of said binary waveform is between 1 milliseconds and 5 seconds.

6. The method of claim 1, wherein the gap time between each pairing of said primary pulse and said secondary pulse is between 10 μ

seconds and 2.5 seconds.

7. The method of claim 1, wherein said determining said difference comprises subtracting said secondary voltammogram from said primary voltammogram.

8. A method for assessing a concentration of an analyte present within an environment, wherein said method comprises:

(a) obtaining a primary voltammogram and a secondary voltammogram, wherein said primary voltammogram was determined from voltammetric data for a primary pulse of a binary waveform, and wherein said secondary voltammogram was determined from voltammetric data for a secondary pulse of said binary waveform,

(b) determining a difference between said primary voltammogram and said secondary voltammogram, wherein said difference provides an indication about said concentration, and

(c) providing information about said difference or said concentration for display to a user.

9. The method of claim 8, wherein said analyte is a chemical capable of being measured by electrochemistry.

10. The method of claim 8, wherein said environment is brain tissue in vivo.

11. The method of claim 8, wherein said environment is human brain tissue in vivo.

12. The method of claim 8, wherein the repetition time of said binary waveform is between 1 milliseconds and 5 seconds.

13. The method of claim 8, wherein the gap time between each pairing of said primary pulse and said secondary pulse is between 10 μ

seconds and 2.5 seconds.

14. The method of claim 8, wherein said determining said difference comprises subtracting said secondary voltammogram from said primary voltammogram.

15. A computer-implemented method for assessing a concentration of an analyte within a sample, wherein said method comprises:

(a) determining, by a computing system, a difference between a primary voltammogram and a secondary voltammogram, wherein said difference provides an indication about said concentration, wherein said primary voltammogram was determined from voltammetric data for a primary pulse of a binary waveform, and wherein said secondary voltammogram was determined from voltammetric data for a secondary pulse of said binary waveform, and

(b) providing information about said difference or said concentration for display to a user.

16. The method of claim 15, wherein said analyte is a chemical capable of being measured by electrochemistry.

17. The method of claim 15, wherein said sample is brain tissue.

18. The method of claim 15, wherein said sample is human brain tissue.

19. The method of claim 15, wherein the repetition time of said binary waveform is between 1 milliseconds and 5 seconds.

20. The method of claim 15, wherein the gap time between each pairing of said primary pulse and said secondary pulse is between 10 μ

seconds and 2.5 seconds.

21. The method of claim 15, wherein said determining said difference comprises subtracting said secondary voltammogram from said primary voltammogram.

22. A computerized system for assessing a concentration of an analyte within a sample, comprising:

(a) a communication port configured to receive voltammetric data for a primary pulse and a secondary pulse of a binary waveform applied to said sample,

(b) one or more computer-readable storage media having recorded thereon instructions that, when executed, determines;

(i) a primary voltammogram and a secondary voltammogram from said data, and

(ii) a difference between said primary voltammogram and said secondary voltammogram, wherein said difference provides an indication about said concentration of said analyte, and

(c) a communication port configured to provide information about said difference or said concentration for display to a user.

23. The computerized system of claim 22, wherein said analyte is a chemical capable of being measured by electrochemistry.

24. The computerized system of claim 22, wherein said sample is brain tissue.

25. The computerized system of claim 22, wherein said sample is brain tissue within a human.

26. The computerized system of claim 22, wherein the repetition time of said binary waveform is between 1 milliseconds and 5 seconds.

27. The computerized system of claim 22, wherein the gap time between each pairing of said primary pulse and said secondary pulse is between 10 μ

seconds and 2.5 seconds.

28. The computerized system of claim 22, wherein said difference comprises a voltammogram determined by subtracting said secondary voltammogram from said primary voltammogram.

29. A tangible, non-transitory computer program product comprising instructions that, when executed, determines:

(a) a primary voltammogram from voltammetric data for a primary pulse of a binary waveform applied to an environment,

(b) a secondary voltammogram from voltammetric data for a secondary pulse of said binary waveform, and

(c) a difference between said primary voltammogram and said secondary voltammogram, wherein said difference provides an indication about a concentration of an analyte within said environment.

30. The computer program product of claim 29, wherein said analyte is a chemical capable of being measured by electrochemistry.

31. The computer program product of claim 29, wherein said environment is brain tissue in vivo.

32. The computer program product of claim 29, wherein said environment is brain tissue within a human.

33. The computer program product of claim 29, wherein the repetition time of said binary waveform is between 1 milliseconds and 5 seconds.

34. The computer program product of claim 29, wherein the gap time between each pairing of said primary pulse and said secondary pulse is between 10 μ

seconds and 2.5 seconds.

35. The computer program product of claim 29, wherein said difference is determined by subtracting said secondary voltammogram from said primary voltammogram.