METHODS OF MAKING CELL CARRIER

• US 20130034669A1
• Filed: 11/02/2011
• Published: 02/07/2013
• Est. Priority Date: 12/16/2010
• Status: Active Grant

• Current Assignee: Global Life Sciences Solutions USA LLC (Danaher Corporation)
• Original Assignee: General Electric Company
• Inventors: Yoon, Yosang, Rubinsztajn, Slawomir, Balch, Gary Stephen, Susarla, Prameela, Caraher, Joel Matthew
• Granted Patent: US 9,453,197 B2
• Time in Patent Office: Days
• US Class Current: 427/570
• CPC Class Codes: B29C 59/04 using rollers or endless belts
• CPC Class Codes: B29C 59/10 by electric discharge treatment
• CPC Class Codes: B29C 59/14 by plasma treatment plasma tubes per se H01J
• CPC Class Codes: C12M 23/20 Material Coatings immunocoatings C12M25/00
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 2535/10 Patterned coating
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

A method of making a carrier for growing cells, including providing a polymer film; embossing a patterned surface one or more sides of the polymer film with an embossing roller; generating a pattern of structured indentations on the polymer film; and discretizing the patterned polymer film into a plurality of portions. The embossing pattern generates relief features on the carrier surface. An alternative method of making a carrier is also provided, including extruding a polymer film; embossing a patterned surface on the polymer film with a roller; generating a pattern of structured indentations on the polymer film; imparting a surface treatment to the film; and discretizing the treated polymer film into a plurality of portions.

Claims

1. A method of making a carrier for growing cells, comprising:

a) providing a polymer film;

b) embossing on one or more sides of the polymer film with an embossing roller comprising patterned surface;

c) generating a pattern of structured indentation on one or more side of the polymer film; and

d) discretizing the patterned polymer film into a plurality of portions.

2. The method of claim 1, further comprising imparting a surface treatment selected from one or more of corona discharge treatment, gas plasma treatment, chemical functionalization or coating.

3. The method of claim 2, further comprising imparting a coating of a thermoresponsive agent, a pH responsive agent, or a combination thereof.

4. The method of claim 2, further comprising imparting a coating of collagen, vitronectin, fibronectin, or laminin.

5. The method of claim 2, further comprising imparting a coating of cytophilic material.

6. The method of claim 1, further comprising heating of the film to soften the film before embossing.

7. The method of claim 6, wherein heating is performed using an IR heater, a quartz heater, a flame, and a combination thereof.

8. The method of claim 1, further comprising cooling of the embossed film.

9. The method of claim 8, wherein cooling is performed by blowing air or other gases, using water bath, using chilled rollers and combinations thereof.

10. The method of claim 1, wherein the embossing roller comprises a hard metal, silicone, teflon or rubber.

11. The method of claim 10, wherein the metal is selected from nickel, copper, steel, stainless steel, and combination thereof.

12. A method of making a carrier for growing cells, comprising:

a) providing a polymer film;

b) embossing on one or more sides of the polymer film with an embossing roller comprising patterned surface;

c) generating a pattern of relief features on one or more side of the polymer film; and

d) discretizing the patterned polymer film into a plurality of portions.

13. The method of claim 12, further comprising imparting a surface treatment selected from one or more of corona discharge treatment, gas plasma treatment, chemical functionalization or coating.

14. The method of claim 13, further comprising imparting a coating of a thermoresponsive agent, a pH responsive agent, or a combination thereof.

15. The method of claim 13, further comprising imparting a coating of collagen, vitronectin, fibronectin, or laminin.

16. The method of claim 13, further comprising imparting a coating of cytophilic material.

17. The method of claim 12, further comprising heating of the film to soften the film before embossing.

18. The method of claim 17, wherein heating is performed using an IR heater, a quartz heater, a flame, and a combination thereof.

19. The method of claim 12, further comprising cooling of the embossed film.

20. The method of claim 19, wherein cooling is performed by blowing air or other gases, using water bath, using chilled rollers and combinations thereof.

21. The method of claim 12, wherein the embossing roller comprises a hard metal, silicone, teflon or rubber.

22. The method of claim 21, wherein the metal is selected from nickel, copper, steel, stainless steel, and combination thereof.

23. A method of making a carrier for growing cells, comprising:

a) extruding a polymer film;

b) embossing on one or more sides of the polymer film with a roller comprising patterned surface;

c) generating a pattern of structured indentation on one or more side of the polymer film;

d) imparting a surface treatment to the film comprising one or more of corona discharge treatment, gas plasma treatment, chemical functionalization or coating; and

e) discretizing the treated polymer film into a plurality of portions.

24. A method of making a carrier for growing cells, comprising:

a) extruding a polymer film;

b) embossing on one or more sides of the polymer film with a roller comprising patterned surface;

c) generating a pattern of one or more relief features on one or more side of the polymer film;

d) imparting a surface treatment to the film comprising one or more of corona discharge treatment, gas plasma treatment, chemical functionalization or coating; and

e) discretizing the treated polymer film into a plurality of portions.

25. The method of claim 24, further comprising imparting a coating of a thermoresponsive agent, a pH responsive agent, or a combination thereof.

26. The method of claim 24, further comprising imparting a coating of collagen, vitronectin, fibronectin, or laminin.

27. The method of claim 24, further comprising imparting a coating of cytophilic material.

28. The method of claim 24, further comprising heating of the film to soften the film before embossing.

29. The method of claim 24, wherein heating is performed using an IR heater, a quartz heater, a flame, and a combination thereof.

30. The method of claim 24, further comprising cooling of the embossed film.

31. The method of claim 30, wherein cooling is performed by blowing air or other gases, using water bath, using chilled rollers and combinations thereof.

32. The method of claim 24, wherein the embossing roller comprises nickel, copper, steel, stainless steel, silicone, teflon or rubber.