Compound Arrays for Sample Profiling

• US 20130079242A1
• Filed: 09/21/2012
• Published: 03/28/2013
• Est. Priority Date: 06/19/2009
• Status: Active Grant

• Current Assignee: Arizona Board of Regents (University of Arizona)
• Original Assignee: Arizona Board of Regents (University of Arizona)
• Inventors: Johnston, Stephen Albert, Stafford, Phillip
• Granted Patent: US 9,709,558 B2
• Time in Patent Office: Days
• US Class Current: 506/9
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: G01N 33/53 Immunoassay; Biospecific binding assay; Materials therefor
• CPC Class Codes: G01N 33/543 with an insoluble carrier for immobilising immunochemicals
• CPC Class Codes: G01N 33/54306 Solid-phase reaction mechanisms
• CPC Class Codes: G01N 33/6845 Methods of identifying protein-protein interactions in protein mixtures
• CPC Class Codes: G01N 33/6854 Immunoglobulins

Abstract

The invention provides arrays of compound for use in profiling samples. The arrays include compounds bind to components of the samples at relatively low affinities. The avidity of compounds binding to components of the samples can be increased by forming arrays such that multivalent components of the samples (e.g., antibodies or cells) can bind to more than one molecule of a compound at the same time. When a sample is applied to an array under such conditions, the compounds of the array bind to component(s) of the sample with significantly different avidities generating a profile characteristic of the sample.

Claims

1-84. -84. (canceled)

85. A method of testing a vaccine, comprising

contacting a blood sample of a subject immunized with a vaccine against a pathogenic microorganism with an array of immobilized different compounds occupying different areas of the array;

detecting a pattern of binding of the sample to the different compounds in the array; and

comparing the pattern of binding to the pattern of binding of one or more reference samples, wherein the reference samples are from subjects who have survived an infection with the virus, similarity of binding profile between the subject and reference samples providing an indication the vaccine is effective against the pathogenic microorganism.

86. The method of claim 85, wherein the subjects have been immunized with a vaccine before exposure to the virus.