Method and Apparatus for Producing a Stabilized Antimicrobial Non-toxic Electrolyzed Saline Solution Exhibiting Potential as a Therapeutic

• US 20130092531A1
• Filed: 12/28/2012
• Published: 04/18/2013
• Est. Priority Date: 10/31/2007
• Status: Active Grant

• Current Assignee: RDG Holdings, Inc.
• Original Assignee: Reoxcyn Discoveries Group
• Inventors: Norton, Verdis, Samuelson, Gary L.
• Granted Patent: US 9,255,336 B2
• Time in Patent Office: Days
• US Class Current: 204/274
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 33/00 Medicinal preparations containing inorganic active ingredients
• CPC Class Codes: A61K 33/14 Alkali metal chlorides; Alkaline earth metal chlorides
• CPC Class Codes: A61K 33/20 Elemental chlorine; Inorganic compounds releasing chlorine
• CPC Class Codes: A61K 33/40 Peroxides
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: C25B 15/02 Process control or regulation

Abstract

An improved method and apparatus for producing a stable, non-toxic, antimicrobial electrolyzed saline solution with a broad range of anti-infective and therapeutic applications. The resulting electrolyzed saline solution exhibits a marked lack of toxicity upon intravenous, aspired, oral or topical application in mammals for therapeutic applications providing a broad platform, including topical disinfection, antimicrobial application, wound treatment, oxidative stress reduction and enhancement of immune function to better detect malfunctioning cells.

Claims

1. An apparatus for producing a stable, non-toxic, antimicrobial electrolyzed saline solution exhibiting anti-infective and immune-enhancing potential as a therapeutic containing regulated amounts of stable reactive oxygen species (ROS), comprising:

a. a container filled with a saline solution having a saline concentration of at least about 0.15%,

b. an inert anode and a spaced apart corresponding inert cathode placed within the saline solution,

c. a temperature regulator for regulating the temperature of the saline solution to maintain a solution temperature sufficient to prevent production of chlorates and regulate relative concentrations of resulting components during electrolysis,

d. circulation means associated with the container for circulating the saline solution to maintain a flow of the saline solution between the anode and cathode,

e. a power source associated with the anode and cathode to apply an effective voltage potential less than about thirty volts between the cathode and the anode sufficient to produce a balanced mixture of chemically reduced and oxidized species including Hypochlorous acid (HOCl), Hypochlorites (OCl⁻

, NaClO), dissolved Oxygen (O₂), Chlorine (Cl₂) between 1 to 200 ppm and Hydrogen (H₂) gas, Hydrogen Peroxide (H₂O₂), Hydrogen ions (H⁺), Hypochloride (ClO) and corresponding amounts of Superoxides (*O₂⁻

, HO₂), Ozone (O₃) between about 1 ppm and about 50 ppm, Activated Hydrogen ions (H⁻

), Chloride ions (Cl⁻

), Hydroxides (NaOH, OH⁻

), Singlet Oxygen (*O₂) and other forms of Reactive Oxygen Species (ROS) (*OCl, *HO⁻

), and wherein total ROS is between about 0.05 ppm and about 50 ppm, utilizing at least one of electron and proton donation, ion and dissolved-gas transport, the temperature, anode and cathode spacing, saline solution circulation rate, and effective voltage combination selected to achieve desired electrolysis efficiencies and stable specie compositions containing stable ROS compounds while preventing production of chlorates.

2. An apparatus for producing a stable, non-toxic, antimicrobial electrolyzed saline solution exhibiting anti-infective and immune-enhancing potential as a therapeutic containing regulated amounts of stable reactive oxygen species (ROS) according to claim 1, wherein the container is fabricated from a biologically compatible material.

3. An apparatus for producing a stable, non-toxic, antimicrobial electrolyzed saline solution exhibiting anti-infective and immune-enhancing potential as a therapeutic containing regulated amounts of stable reactive oxygen species (ROS) according to claim 1, wherein the anode is made of a base metal selected from the group consisting of platinum, niobium, titanium or any metal compatible with platinum bonding and is coated with an outer layer of platinum bonded to the base metal.

4. An apparatus for producing a stable, non-toxic, antimicrobial electrolyzed saline solution exhibiting anti-infective and immune-enhancing potential as a therapeutic containing regulated amounts of stable reactive oxygen species (ROS) according to claim 3, wherein the anode has a cylindrical, or flat (planar) shaped structure.

5. An apparatus for producing a stable, non-toxic, antimicrobial electrolyzed saline solution exhibiting anti-infective and immune-enhancing potential as a therapeutic containing regulated amounts of stable reactive oxygen species (ROS) according to claim 4, wherein the cathode has a cylindrical, or flat (planar) shaped structure and is positioned coaxially or in parallel in relation to the anode.

6. An apparatus for producing a stable, non-toxic, antimicrobial electrolyzed saline solution exhibiting anti-infective and immune-enhancing potential as a therapeutic containing regulated amounts of stable reactive oxygen species (ROS) according to claim 5, wherein the cathode is made of a base metal selected from the group consisting of platinum, niobium, titanium or any metal compatible with platinum bonding and is plated with an outer layer of platinum bonded to the base metal.

7. An apparatus for producing a stable, non-toxic, antimicrobial electrolyzed saline solution exhibiting anti-infective and immune-enhancing potential as a therapeutic containing regulated amounts of stable reactive oxygen species (ROS) according to claim 1, wherein the spacing between the cathode and the anode is less than one inch and is dependent upon ion transfer rates and electric fields to achieve desired electrolysis efficiencies to produce different varieties of solution components all containing stable ROS compounds.

8. An apparatus for producing a stable electrolyzed saline solution for treating a human or warm-blooded animal, comprising:

a. a container filled with a saline solution having a concentration between about 0.15% and about 1.0% by weight;

b. an inert anode and an inert cathode placed within the container associated with a power source configured to apply an effective voltage potential less than about thirty volts between the cathode and the anode to electrolyze the saline solution and produce a first target mixture of chemically reduced and oxidized molecules;

c. a known biological system including a second target mixture of chemically reduced and oxidized molecules,

wherein the first target mixture is configured to mirror the second target mixture.

9. The apparatus of claim 8, wherein the first target mixture and the second target mixture are measured using a fluorospectrometer configured to measure concentrations of reactive oxygen species.

10. The apparatus of claim 9, wherein the concentrations are measured using at least one fluorescent dye selected from R-phycoerytherin, hydroxyphenyl fluorescein, and aminophenyl fluorescein.

11. The apparatus of claim 8, further comprising a regulator for regulating temperature of the saline solution to maintain a solution temperature sufficient to prevent production of chlorates.

12. The apparatus of claim 8, further comprising a circulation means associated with the container.

13. The apparatus of claim 8, wherein the first target mixture of chemically reduced and oxidized molecules includes at least one of HOCl, OCl⁻

, NaClO, O₂, Cl₂, H₂, H₂O₂, H⁺, ClO, *O₂⁻

, HO₂, O₃, H⁻

, Cl⁻

, NaOH, OH⁻

, *O₂, *OCl, or *HO⁻

.

14. The apparatus of claim 8, wherein the container is fabricated from a biologically compatible material.

15. The apparatus of claim 8, wherein the anode is made of a base metal selected from platinum, niobium, titanium, a combination thereof, or any metal compatible with platinum bonding and is coated with an outer layer of platinum.

16. The apparatus of claim 8, wherein the anode has a cylindrical or flat shaped structure.

17. The apparatus of claim 8, wherein the cathode has a cylindrical or flat shaped structure.

18. The apparatus of claim 8, wherein the cathode is made of a base metal selected from platinum, niobium, titanium, a combination thereof, or any metal compatible with platinum bonding, and is plated with an outer layer of platinum.

19. The apparatus of claim 8, wherein a space between the cathode and the anode is less than one inch.

20. The apparatus of claim 19, wherein the space is dependent upon ion transfer rates and electric fields.