CLOSED SYSTEM SEPARATION OF ADHERENT BONE MARROW STEM CELLS FOR REGENERATIVE MEDICINE APPLICATIONS
First Claim
1. A method for isolating and processing bone marrow derived stem cells comprising the steps of:
- (a) collecting a biological sample containing adherent bone marrow stem cells in a receptacle with interior walls coated with a cell-adherent substrate;
(b) incubating the bone marrow cells on the adherent substrate so that a layer of adherent bone marrow stem cells adheres to the substrate;
(c) washing any non-adherent cells from said substrate; and
(d) collecting said bone marrow stem cell layer.
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Accused Products
Abstract
A method for isolating and processing bone marrow derived stem cells, including the steps of: (a) collecting a biological sample containing adherent bone marrow stem cells in a receptacle with interior walls coated with a cell-adherent substrate; (b) incubating the bone marrow cells on the adherent substrate so that a layer of adherent bone marrow stem cells adheres to the substrate; (c) washing any non-adherent cells from the substrate; and (d) collecting the bone marrow stem cell layer. Isolation kits and use of bone marrow cells harvested for cell therapies are also described.
18 Citations
34 Claims
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1. A method for isolating and processing bone marrow derived stem cells comprising the steps of:
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(a) collecting a biological sample containing adherent bone marrow stem cells in a receptacle with interior walls coated with a cell-adherent substrate; (b) incubating the bone marrow cells on the adherent substrate so that a layer of adherent bone marrow stem cells adheres to the substrate; (c) washing any non-adherent cells from said substrate; and (d) collecting said bone marrow stem cell layer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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24. A stem cell isolation kit, comprising:
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(a) a first cell collection bag containing a predetermined quantity of a plasma blood volume expander; (b) a second cell collection bag comprising an interior surface coated with a cell-adherent surface layer and containing a predetermined quantity of growth medium; and (c) sterile connection means for transferring the contents of said first bag to said second bag. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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Specification