DETECTION DEVICE AND DETECTION METHOD FOR INTERMOLECULAR INTERACTION

• US 20130122607A1
• Filed: 05/18/2011
• Published: 05/16/2013
• Est. Priority Date: 07/27/2010
• Status: Active Grant

• Current Assignee: Konica Minolta Inc.
• Original Assignee: Konica Minolta Inc.
• Inventors: Kashiwazaki, Osamu
• Granted Patent: US 8,681,338 B2
• Time in Patent Office: Days
• US Class Current: 436/501
• CPC Class Codes: G01N 2021/7773 Reflection
• CPC Class Codes: G01N 2021/7779 interferometric
• CPC Class Codes: G01N 21/55 Specular reflectivity
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator

Abstract

In order to improve the detection accuracy of a reflection spectrum, a detection device for intermolecular interaction is provided with a detector (10) which has a ligand (16), a white light source (20) which emits white light, a spectroscope (30) which detects the spectral intensity of received light, a light transmission unit (40) which has a first light transmission path (41) for transmitting the white light from the white light source to the detector, a second light transmission path (42) for transmitting reflected light of the white light from the detector to the spectroscope, and a third light transmission path (43) for transmitting the white light from the white light source to the spectroscope, a switching unit (80) which performs switching between a reflected light receiving state in which the transmission of the reflected light of the white light in the detector to the spectroscope via the first and second light transmission paths is enabled and a white light receiving state in which the transmission of the white light from the white light source to the detector via the third light transmission path is enabled, and a control unit (50) which controls the switching unit and the spectroscope to perform control for detecting the spectral intensities in the white light receiving state and the reflected light receiving state, respectively.

Claims

1. A detection device for an intermolecular interaction, comprising:

a detector having a ligand;

a white light source emitting white light;

a spectrometer to detect a spectral intensity of received light;

a light transmission unit including a first optical transmission path for transmitting the white light from the white light source to the detector, a second optical transmission path for transmitting reflected light of the white light from the detector to the spectrometer and a third optical transmission path for transmitting the white light from the white light source to the spectrometer;

a switching unit switching between a reflected light receiving state which allows the reflected light of the white light at the detector to be transmitted to the spectrometer via the second optical transmission paths and a white light receiving state which allows the white light from the white light source to be transmitted to the spectrometer via the third optical transmission path; and

a controller controlling the switching unit and the spectrometer to detect the spectral intensities in the white light receiving state and in the reflected light receiving state.

2. The detection device for an intermolecular interaction according to claim 1, further comprising a calculation unit to estimate a reflection spectrum through calculation of a reflectance for every predetermined wavelength range on a basis of the spectral intensities of the white light and the reflected light obtained by the control of the controller.

3. The detection device for an intermolecular interaction according to claim 1, wherein the switching unit is a shutter mechanism selectively switchable between an optically shielded state of a light transmission via the first optical transmission path and an optically shielded state of a light transmission via the third optical transmission path with a shielding member, the position of the shielding member being movable.

4. The detection device for an intermolecular interaction according to claim 1, wherein the switching unit is a liquid crystal filter selectively switchable between an optically shielded state of a light transmission via the first optical transmission path and an optically shielded state of a light transmission via the third optical transmission path.

5. A detection method for detecting an intermolecular interaction using a detection device comprising a detector having a ligand, a white light source emitting white light, a spectrometer detecting spectral intensity of received light, a light transmission unit including a first optical transmission path for transmitting the white light from the white light source to the detector, a second optical transmission path for transmitting reflected light of the white light from the detector to the spectrometer and a third optical transmission path for transmitting the white light from the white light source to the spectrometer, the method comprising:

a first detection step of receiving the reflected light of the white light from the detector via the first and the second optical transmission paths and detecting the spectral intensity of the reflected light by the spectrometer;

a second detection step of receiving the white light from the white light source via the third optical transmission path either before or after the first detection step and detecting the spectral intensity of the white light by the spectrometer; and

a calculation step of estimating a reflection spectrum by calculating a reflectance for every predetermined wavelength range on a basis of the spectral intensities of the white light and the reflected light obtained in the first detection step and the second detection step.