Programmable Oligonucleotide Synthesis
First Claim
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1. A method of preparation of synthetic nucleic acids, in particular of nucleic acid double strands, comprising the steps:
- a. preparation of a multiplicity of different nucleic acid fragments by solid-phase synthesis, preferably at various positions of a common solid support, andb. joining together of at least two nucleic acid fragments from (a) by binding to one another or by covalent linkage,characterized in that at least some nucleic acid fragments, which have a high AT content, are used in an increased amount relative to other fragments for step (b).
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Abstract
The invention relates to methods and devices for preparing synthetic nucleic acids.
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6 Claims
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1. A method of preparation of synthetic nucleic acids, in particular of nucleic acid double strands, comprising the steps:
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a. preparation of a multiplicity of different nucleic acid fragments by solid-phase synthesis, preferably at various positions of a common solid support, and b. joining together of at least two nucleic acid fragments from (a) by binding to one another or by covalent linkage, characterized in that at least some nucleic acid fragments, which have a high AT content, are used in an increased amount relative to other fragments for step (b). - View Dependent Claims (2, 3, 4)
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5. A polypeptide with defined properties for use as a pharmaceutically active substance and/or for use in the production of a pharmaceutically active substance, produced by synthesis of synthetic nucleic acids with the sequence encoding the polypeptide, in particular of nucleic acid double strands, comprising the steps:
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a. preparation of a multiplicity of different nucleic acid fragments by solid-phase synthesis, preferably at various positions of a common solid support, b. joining together of at least two nucleic acid fragments from (a) by binding to one another, characterized in that at least some nucleic acid fragments, which have a high AT content, are used in an increased amount relative to other fragments for step (b), and where the method further comprises; c. production of the polypeptide using said sequence. - View Dependent Claims (6)
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Specification