METHODS FOR THE PRODUCTION OF IPS CELLS USING NON-VIRAL APPROACH

• US 20130189778A1
• Filed: 03/11/2013
• Published: 07/25/2013
• Est. Priority Date: 06/04/2008
• Status: Active Grant

• Current Assignee: FUJIFILM Cellular Dynamics, Inc. (Fujifilm Holdings Corporation)
• Original Assignee: Cellular Dynamics International, Inc. (Fujifilm Holdings Corporation)
• Inventors: MACK, Amanda
• Granted Patent: US 9,644,184 B2
• Time in Patent Office: Days
• US Class Current: 435/366
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 15/85 for animal cells
• CPC Class Codes: C12N 2501/602 Sox-2
• CPC Class Codes: C12N 2501/603 Oct-3/4
• CPC Class Codes: C12N 2501/604 Klf-4
• CPC Class Codes: C12N 2501/605 Nanog
• CPC Class Codes: C12N 2501/606 c-Myc
• CPC Class Codes: C12N 2501/608 Lin28
• CPC Class Codes: C12N 2510/00 Genetically modified cells
• CPC Class Codes: C12N 2710/16222 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2800/107 for mammalian
• CPC Class Codes: C12N 2800/108 episomal vectors
• CPC Class Codes: C12N 2800/24 Vectors characterised by the absence of particular element, e.g. selectable marker, viral origin of replication
• CPC Class Codes: C12N 2820/60 from viruses
• CPC Class Codes: C12N 2840/105 enhancing translation
• CPC Class Codes: C12N 2840/206 having multiple IRES
• CPC Class Codes: C12N 5/0696 Artificially induced pluripotent stem cells, e.g. iPS

Abstract

Methods and composition of induction of pluripotent stem cells and other desired cell types are disclosed. For example, in certain aspects methods for generating essentially vector-free induced pluripotent stem cells are described. Furthermore, the invention provides induced pluripotent stem cells and desired cell types essentially free of exogenous vector elements with the episomal expression vectors to express differentiation programming factors.

Claims

1. A human iPS cell population that is essentially free of exogenous retroviral elements, the cell population comprising the genome of a selected human individual.

3. The iPS cell population of claim 1, wherein the iPS cell population is essentially free of exogenous viral vector elements.

2. A human iPS cell population comprising cells whose genome is derived from a terminally differentiated human cell and essentially free of exogenous retroviral elements.

40. The iPS cell population of claim 2, wherein the iPS cell population is essentially free of exogenous viral vector elements.

4-39. -39. (canceled)